RELATIVE ROLES OF PITUITARY FOLLICLE CELLS AND PROGESTERONE IN INDUCTION OF OOCYTE MATURATION IN RANA PIPIENS
RELATIVE ROLES OF PITUITARY FOLLICLE CELLS AND PROGESTERONE IN INDUCTION OF OOCYTE MATURATION IN RANA PIPIENS
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DOI：
10.1002/jez.1401660309
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发表时间：
1967-01-01
影响因子：
--
通讯作者：
MASUI, Y
中科院分区：
文献类型：
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作者：
MASUI, Y
从成熟蛙（Ranapipiens）卵巢中获得的卵母细胞，在去除卵泡被膜后，观察了卵母细胞对垂体激素的反应。当人工或经胶原酶处理去除卵泡膜后，明显裸露的卵母细胞仍能通过生殖泡破裂和极体形成对垂体激素产生反应。然而，当用EDTA或无钙镁溶液处理后去除卵泡包膜时，卵母细胞的反应性完全丧失。显微镜检查显示，除非使用不含Ca‐Mg‐的溶液或EDTA，否则滤泡细胞的去除不完全。完全去除卵泡细胞会阻止卵母细胞对垂体激素的反应，而且，在存在大量卵巢组织的情况下，用垂体激素溶液处理与先前分离的卵泡包膜一起包装的裸卵母细胞时，显示出成熟反应。不能对垂体激素产生反应的裸卵母细胞，仍然可以对孕酮产生完全反应，进行成熟反应。事实上，单独用孕酮处理可以在完整的卵巢组织内引起生殖囊泡破裂和极体形成。对孕酮有反应的卵母细胞总是留在卵泡被膜内，用核移植的方法检测孕酮处理后卵母细胞的成熟程度。当从囊胚或早期原肠胚细胞获得的细胞核植入处理过的卵母细胞时，仍在卵泡包膜内，发生了正常的卵裂。这些结果表明，垂体促性腺激素作用于卵泡细胞，刺激它们释放一种激素，直接作用于卵母细胞，诱导成熟。
The responses of ovarian oocytes obtained from mature frogs (Rana pipiens) to pituitary hormone were examined after removal of the follicular envelope. When the follicular envelope was removed manually or after treatment with collagenase, the apparently naked oocyte can still respond to pituitary hormone by germinal vesicle breakdown and by polar body formation. However, when the follicular envelope was removed after treatment with EDTA or Ca‐Mg‐free solution, the responsiveness of the oocyte was completely lost. Microscopic examination revealed that removal of the follicular cells was incomplete unless Ca‐Mg‐free solutions or EDTA were used. Complete removal of follicle cells prevents the oocyte from responding to pituitary hormone.Moreover, the naked oocytes packed together with previously separated follicular envelopes showed maturation responses when treated with pituitary hormone solution in the presence of large amounts of ovarian tissue. Naked oocytes which are unable to respond to pituitary hormone can nevertheless full respond to progesterone to undergo maturation reactions. In fact, germinal vesicle berakdown and polar body formation were brought about within intact ovarian tissue by treatment with progesterone alone. Oocytes responding to progesterone alone always remained within the follicular envelope.The degree of maturation of the oocytes after progesterone treatment was tested by means of nuclear transplantation. When nuclei obtained from blastula or early gastrula cells were implanted into treated oocytes, still within the follicular envelope, normal cleavage occurred. These results indicate that pituitary gonadotropin acts on the follicle cells to stimulate them to release a hormone that directly acts on the oocyte to induce maturation.