Controlled vesicle self-assembly in microfluidic channels with hydrodynamic focusing
Controlled vesicle self-assembly in microfluidic channels with hydrodynamic focusing
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DOI：
10.1021/ja0318030
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发表时间：
2004-03-10
影响因子：
15
通讯作者：
Locascio, LE
中科院分区：
文献类型：
--
作者：
Jahn, A;Vreeland, WN;Locascio, LE
传统的脂质体制备方法基于本体相的混合，导致形成过程中化学和/或机械条件不均匀；因此，脂质体的尺寸和层状通常是多分散的。在这里，我们展示了脂质体的形成，该脂质体将试剂封装在连续两相流微流体网络中，通过控制液体流速精确控制 100 至 300 nm 的尺寸。我们证明，通过以微流体形式创建在脂质体长度范围内均匀且可控的溶剂-水界面区域，我们可以促进脂质体尺寸和多分散性的精细控制。
Traditional liposome preparation methods are based on mixing of bulk phases, leading to inhomogeneous chemical and/or mechanical conditions during formation; hence liposomes are often polydisperse in size and lamellarity. Here we show the formation of liposomes that encapsulate reagents in a continuous two-phase flow microfluidic network with precision control of size from 100 to 300 nm by manipulation of liquid flow rates. We demonstrate that by creating a solvent−aqueous interfacial region in a microfluidic format that is homogeneous and controllable on the length scale of a liposome, we can facilitate the fine control of liposome size and polydispersity.