Germ Cell-Intrinsic and -Extrinsic Factors Govern Meiotic Initiation in Mouse Embryos
Germ Cell-Intrinsic and -Extrinsic Factors Govern Meiotic Initiation in Mouse Embryos
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DOI：
10.1126/science.1166340
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发表时间：
2008-12-12
期刊：
影响因子：
56.9
通讯作者：
Page, David C.
中科院分区：
文献类型：
--
作者：
Lin, Yanfeng;Gill, Mark E.;Page, David C.
视黄酸 (RA) 是哺乳动物生殖细胞减数分裂起始的重要外源诱导剂。然而，RA 在哺乳动物发育中的作用太广泛，无法单独解释减数分裂起始的细胞类型和时间特异性。我们考虑了与酵母的相似之处，其中外部和内部因素结合起来限制减数分裂的起始。我们证明，在小鼠胚胎中，外在因素和内在因素共同调节减数分裂的起始。小鼠 RNA 结合蛋白 DAZL 由迁移后生殖细胞表达，是一个关键的内在因子，使这些细胞能够响应 RA 启动减数分裂。在短暂的发育窗口内，XX 和 XY 胚胎中表达 Dazl 的生殖细胞主动获得将 RA 解释为减数分裂诱导信号的能力。
Retinoic acid ( RA) is an essential extrinsic inducer of meiotic initiation in mammalian germ cells. However, RA acts too widely in mammalian development to account, by itself, for the cell- type and temporal specificity of meiotic initiation. We considered parallels to yeast, in which extrinsic and intrinsic factors combine to restrict meiotic initiation. We demonstrate that, in mouse embryos, extrinsic and intrinsic factors together regulate meiotic initiation. The mouse RNA- binding protein DAZL, which is expressed by postmigratory germ cells, is a key intrinsic factor, enabling those cells to initiate meiosis in response to RA. Within a brief developmental window, Dazl- expressing germ cells in both XX and XY embryos actively acquire the ability to interpret RA as a meiosis- inducing signal.