Caged RNA: photo-control of a ribozyme reaction
Caged RNA: photo-control of a ribozyme reaction
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DOI：
10.1093/nar/26.13.3173
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发表时间：
1998-07-01
影响因子：
14.9
通讯作者：
MacMillan, AM
中科院分区：
文献类型：
--
作者：
Chaulk, SG;MacMillan, AM
我们在这里报道了第一个光化学控制的核酶反应，通过位点特异性修饰的2'-羟基亲核试剂在锤头系统具有笼化功能。在天然条件下，快速激光光解O-（2-硝基苯）笼基启动了一个高效和准确的锤头催化的底物RNA裂解。以这种方式将反应性功能或识别元件关在笼子里的RNA将是探测RNA反应性和动力学的有用工具。
We report here the first photo-chemical control of a ribozyme reaction by the site-specific modification of the 2'-hydroxyl nucleophile in the hammerhead system with a caging functionality. Rapid laser photolysis of the O-(2-nitrobenzyl) caging group initiates an efficient and accurate hammerhead-catalyzed cleavage of substrate RNA under native conditions. RNAs in which reactive functionalities or recognition elements are caged in this manner will be useful tools to probe RNA reactivity and dynamics.