Cloning and Characterization of a GABA Receptor from Plutella xylostella (Lepidoptera: Plutellidae)
Cloning and Characterization of a GABA Receptor from Plutella xylostella (Lepidoptera: Plutellidae)
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DOI：
10.1603/0022-0493-101.6.1888
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发表时间：
2008-12
期刊：
影响因子：
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通讯作者：
Xiao-mao Zhou;Qing-jun Wu;Y. Zhang;L. Bai;Xiong-ying Huang
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文献类型：
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作者：
Xiao-mao Zhou;Qing-jun Wu;Y. Zhang;L. Bai;Xiong-ying Huang
摘要从小菜蛾Plutella xylostella（L.）（鳞翅目：小菜蛾科）（GenBank登录号EF 156251）。该亚基基因编码483个氨基酸的多肽，与DmRdl亚基（U 02042）（抗狄氏剂的黑腹果蝇）显示84%的序列同一性。当在非洲爪蟾卵母细胞中表达时，该亚基组装成功能性同源复合物，由GABA和阿维菌素以剂量依赖性方式激活。GABA的EC 50值为0.49 mM（0.41-0.58）（n = 5）。然而，对阿维菌素的反应非常稳健，EC 50为4.85 μM（4.02-5.89）（n = 6），表明阿维菌素在激活氯电流方面比GABA强100倍以上。结果表明，该亚基是至关重要的功能通道的形成，并包含阿维菌素的结合位点。
Abstract A full-length cDNA, with an open reading frame (ORF) of 1,449 bp, encoding a subunit of the γ-aminobutyric acid (GABA)-activated chloride channel was isolated from Plutella xylostella (L.) (Lepidoptera: Plutellidae) (GenBank accession no. EF156251). The subunit gene encoded a 483-amino acid polypeptide that showed 84% sequence identity with DmRdl subunit (U02042) (Drosophila melanogaster resistant to dieldrin). When expressed in Xenopus laevis oocytes, the subunit assembled as a functional homomeric complex activated by GABA and abamectin in a dose-dependent manner. The EC50 value of GABA was 0.49 mM (0.41–0.58) (n = 5). However, the responses to abamectin were very robust, with an EC50 of 4.85 μM (4.02–5.89) (n = 6), indicating that abamectin was >100-fold more potent in activating chloride currents than GABA. The results suggest that this subunit is vital to the formation of a functional channel and contains the binding site of abamectin.