A complex of the Srb8,-9,-10, and-11 transcriptional regulatory proteins from yeast
A complex of the Srb8,-9,-10, and-11 transcriptional regulatory proteins from yeast
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DOI：
10.1074/jbc.m207195200
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发表时间：
2002-11-15
影响因子：
4.8
通讯作者：
Kornberg, RD
中科院分区：
文献类型：
--
作者：
Borggrefe, T;Davis, R;Kornberg, RD
酵母的Srb8， -9， -10和-11蛋白已被分离为一个离散的，化学计量的复合物。分离的复合物磷酸化RNA聚合酶11最大亚基的丝氨酸2和5的c端结构域（CTD）。除了先前报道的Srb10和srb11的人类同源物外，我们还鉴定了Srb8和Srb9的人类同源物TRAP230/ARC240和TRAP240/ARC250，这表明整个Srb8/9/10/11复合物从酵母到人类是保守的。
The Srb8, -9, -10, and -11 proteins of yeast have been isolated as a discrete, stoichiometric complex. The isolated complex phosphorylates the C-terminal domain (CTD) of the largest subunit of RNA polymerase 11 at serines 2 and 5. In addition to the previously reported human homologs of Srb10 and 11, we have identified TRAP230/ARC240 and TRAP240/ARC250 as the human homologs of Srb8 and Srb9, showing the entire Srb8/9/10/11 complex is conserved from yeast to humans.