Presentation and detection of azide functionality in bacterial cell surface proteins
Presentation and detection of azide functionality in bacterial cell surface proteins
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DOI：
10.1021/ja047629c
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发表时间：
2004-09-01
影响因子：
15
通讯作者：
Tirrell, DA
中科院分区：
文献类型：
--
作者：
Link, AJ;Vink, MKS;Tirrell, DA
描述了一种改进的铜催化在细菌细胞表面形成三氮唑的方法。在用叠氮高丙氨酸(2)处理的细胞中加入高纯度的溴化亚铜，细胞表面的标记比以前观察到的多约10倍。这种高活性催化剂可以检测细胞表面蛋白中的蛋氨酸类似物叠氮丙氨酸(1)、叠氮丙氨酸(3)和叠氮丙氨酸(4)。叠氮丙氨酸此前被认为在细胞蛋白质合成机制方面是沉默的。
An improved protocol for copper-catalyzed triazole formation on the bacterial cell surface is described. Addition of highly pure CuBr to cells treated with azidohomoalanine (2) leads to ca. 10-fold more extensive cell surface labeling than previously observed. This highly active catalyst allows detection of the methionine analogues azidoalanine (1), azidonorvaline (3), and azidonorleucine (4) in cell surface proteins. Azidoalanine was previously believed to be silent with regard to the cellular protein synthesis machinery.