A Fibrin Site‐Specific Nanoprobe for Imaging Fibrin‐Rich Thrombi and Preventing Thrombus Formation in Venous Vessels
A Fibrin Site‐Specific Nanoprobe for Imaging Fibrin‐Rich Thrombi and Preventing Thrombus Formation in Venous Vessels
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纤维蛋白位点特异性纳米探针，用于对富含纤维蛋白的血栓进行成像并防止静脉血管中血栓形成
DOI：
10.1002/adma.202109955
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发表时间：
2022
影响因子：
29.4
通讯作者：
Xing
中科院分区：
文献类型：
--
作者：
Yixin Zhong;Man Ye;Liandi Huang;Liu Hu;Fangzhou Li;Qiankun Ni;J. Zhong;Hongyun Wu;Fengfei Xu;Jie Xu;Xiao;Zhigang Wang;Haitao Ran;Yunzhu Wu;D. Guo;Xing
静脉血栓栓塞症（VTE）是一个普遍的公共卫生问题在世界范围内。在治疗前，时空准确的血栓检测是必不可少的。然而，在目前可用的成像技术下，这是具有挑战性的。本文介绍了一种新型纤维蛋白特异性纳米探针（NP）的开发，该探针基于聚（乳酸-乙醇酸共聚物）与五肽Cys−Arg−Glu−Lys−Ala（CREKA）的缀合，用于体内选择性和半定量成像。通过整合Fe 3 O 4和近红外荧光染料（IR 780），NP可以作为一个高灵敏度的传感器，用于直接分析体内血栓。纤维蛋白特异性NP将富含纤维蛋白的血栓与富含胶原蛋白或富含红细胞的血栓区分开来，这对于未来的个体化治疗策略可能是有益的。此外，用富马酸酮替芬负载NP导致肥大细胞脱粒抑制，因此，NP可以预防血栓形成而没有过度出血的风险。因此，使用纤维蛋白特异性NP可能是未来检测和预防易感人群中VTE的安全替代方法。
Venous thromboembolism (VTE) is a prevalent public health issue worldwide. Before treatment, spatiotemporally accurate thrombus detection is essential. However, with the currently available imaging technologies, this is challenging. Herein, the development of a novel fibrin‐specific nanoprobe (NP) based on the conjugation of poly(lactic‐co‐glycolic acid) with the pentapeptide Cys−Arg−Glu−Lys−Ala (CREKA) for selective and semiquantitative imaging in vivo is presented. By integrating Fe3O4 and NIR fluorochrome (IR780), the NP can function as a highly sensitive sensor for the direct analysis of thrombi in vivo. The fibrin‐specific NP distinguishes fibrin‐rich thrombi from collagen‐rich or erythrocyte‐rich thrombi, which can be beneficial for future individually tailored therapeutic strategy. Furthermore, loading NPs with the ketotifen fumarate results in mast cell degranulation inhibition, and hence, NPs can prevent thrombosis without the risk of excessive bleeding. Thus, the use of fibrin‐specific NPs may serve as a safe alternative approach for the detection and prevention of VTEs in susceptible populations in the future.