EFFECTS OF INTRATHECAL ADMINISTRATION OF NEUROPEPTIDES ON A SPINAL NOCICEPTIVE REFLEX IN THE RAT - VIP, GALANIN, CGRP, TRH, SOMATOSTATIN AND ANGIOTENSIN-II
EFFECTS OF INTRATHECAL ADMINISTRATION OF NEUROPEPTIDES ON A SPINAL NOCICEPTIVE REFLEX IN THE RAT - VIP, GALANIN, CGRP, TRH, SOMATOSTATIN AND ANGIOTENSIN-II
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DOI：
10.1016/0143-4179(88)90024-8
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发表时间：
1988-01-01
期刊：
影响因子：
2.9
通讯作者：
HENRY, JL
中科院分区：
文献类型：
--
作者：
CRIDLAND, RA;HENRY, JL
本研究探讨了鞘内给药所选肽对大鼠伤害性反应的影响。通过长期植入的导管将每种肽递送至L5椎骨水平。在甩尾试验中，VIP（0.65-6.5 nmole）在注射后1至6-16 min产生反应时间（RT）的剂量依赖性降低; 6.5 nmole在注射后1 min将RT降低至对照值的37%。甘丙肽（0.65-6.5纳摩尔）在1分钟和6分钟时产生反应时间的剂量依赖性增加;在高剂量下，许多大鼠未能甩尾。CGRP（6.5纳摩尔）产生了一个小的，短暂的RT下降到73%的控制值在1分钟; 3.25纳摩尔没有影响。CSF和6.5 nmol生长抑素、TRH和血管紧张素II均无效。在高剂量的甘丙肽和CGRP下，大鼠对尾部的无害触摸发声，如对于P物质所报道的。因此，在6.5纳摩尔的VIP、甘丙肽、CGRP或P物质后，将Von Frey毛发施加到尾部。在注射后30秒，在给予甘丙肽的所有大鼠和给予CGRP或P物质的一些大鼠中观察到响应于先前无害的压力刺激的发声，作用持续4-8 min，VIP、CSF无明显作用。这些结果表明，VIP，甘丙肽，降钙素基因相关肽和P物质可能作为兴奋剂在伤害性通路和特定的肽可能在不同类型的疼痛模式中发挥作用; VIP在热，甘丙肽在机械和P物质和降钙素基因相关肽两者。
The present study examines the effects of intrathecal administration of selected peptides on nociceptive responses in the rat. Each peptide was delivered via a chronically implanted catheter to the L5 vertebral level. In the tail flick test, VIP (0.65-6.5 nmoles) produced a dose-dependent decrease in reaction time (RT) from 1 to 6-16 min after injection; 6.5 nmoles decreased RT to 37% of control value at 1 min after injection. Galanin (0.65-6.5 nmoles) produced a dose-dependent increase in reaction time at 1 and 6 min; at high doses, many of the rats failed to flick the tail. CGRP (6.5 nmoles) produced a small, transient decrease in RT to 73% of control values at 1 min; 3.25 nmoles were without effect. CSF and 6.5 nmoles of somatostatin, TRH and angiotensin II were without effect. At high doses of galanin and CGRP, rats vocalized to innocuous touch of the tail, as reported for substance P. Von Frey hairs were thus applied to the tail after 6.5 nmoles of VIP, galanin, CGRP or substance P. Vocalization in response to a previously innocuous pressure stimulus was observed at 30s after injection in all rats given galanin and some rats given CGRP or substance P, the effect lasted 4-8 min. VIP and CSF had no effect. These results suggest that VIP, galanin, CGRP and substance P may act as excitatory agents in nociceptive pathways and that specific peptides may function in the different types of pain modalities; VIP in thermal, galanin in mechanical and substance P and CGRP in both.