Ostrich produce cross-reactive neutralization antibodies against pandemic influenza virus A/H1N1 following immunization with a seasonal influenza vaccine
Ostrich produce cross-reactive neutralization antibodies against pandemic influenza virus A/H1N1 following immunization with a seasonal influenza vaccine
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DOI：
10.3892/etm.2010.180
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发表时间：
2011-01-01
影响因子：
2.7
通讯作者：
Tsukamoto, Yasuhiro
中科院分区：
文献类型：
--
作者：
Adachi, Kazuhide;Takama, Kentaro;Tsukamoto, Yasuhiro
2009年爆发的流感被发现是由一种被称为2009年甲型H1N1流感大流行的新型流感病毒株引起的。在任何年龄组的人群中，接种最新的季节性流感疫苗对2009年甲型H1N1流感病毒的交叉反应性抗体应答很少或没有诱导。因此，大多数人对这种病原体的免疫力较低，从而导致感染在世界范围内传播，产生所谓的“流行病”。本报告介绍了一项重要发现，即用季节性流感疫苗免疫雌性鸵鸟后，鸵鸟蛋产生了针对大流行性流感病毒A/H1N1的交叉反应抗体。这种简单的方法可以使一只雌性鸵鸟产生大量的流感病毒抗体。酶联免疫吸附试验（ELISA）和免疫细胞化学结果表明，所制备的抗体与甲型H1N1流感病毒、季节性甲型H1N1流感病毒、季节性甲型H3 N2流感病毒和季节性B流感病毒均具有较强的交叉反应性。鸵鸟抗体也能抑制该大流行株诱导的红细胞聚集活性。此外，在与鸵鸟抗体的共培养物中，感染大流行病毒对MDCK细胞的细胞病理学效应明显受到抑制，从而表明细胞中病毒感染性的中和。总之，在用季节性流感病毒疫苗免疫的雌性鸵鸟所产的鸵鸟蛋中成功地产生了针对大流行性流感病毒A/H1N1 2009的交叉反应中和抗体。
An outbreak of influenza in 2009 was found to be caused by a novel strain of influenza virus designated as pandemic influenza A/H1N1 2009. Vaccination with recent seasonal influenza vaccines induced little or no cross-reactive antibody response to the pandemic influenza virus A/H1N1 2009 in any age group in human populations. Accordingly, most people had low immunity against this pathogen, thus resulting in the worldwide spread of the infection to produce a so-called 'pandemic'. This report presents the important finding that ostrich eggs generate cross-reactive antibodies to the pandemic influenza virus A/H1N1 following immunization of female ostrich with a seasonal influenza vaccine. This simple method produced a large amount of antibodies against influenza viruses by one female ostrich. An enzyme-linked immunosorbent assay (ELISA) and immunocytochemistry indicated that the ostrich antibodies possessed strong cross-reactivity to the pandemic A/H1N1 as well as to the seasonal A/H1N1, A/H3N2 and B viruses. The hemaggregation activities of erythrocytes induced by this pandemic strain were also inhibited by the ostrich antibodies. In addition, the cytopathological effects of infection with a pandemic virus on MDCK cells were clearly inhibited in co-cultures with the ostrich antibodies, thereby indicating the neutralization of viral infectivity in the cells. In conclusion, cross-reactive neutralization antibodies against pandemic influenza virus A/H1N1 2009 were successfully generated in ostrich eggs produced by females immunized with seasonal influenza viral vaccine.