Host Specificity of Endophytic Fungi from Stem Tissue of Nature Farming Tomato (Solanum lycopersicum Mill.) in Japan
Host Specificity of Endophytic Fungi from Stem Tissue of Nature Farming Tomato (Solanum lycopersicum Mill.) in Japan
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DOI：
10.3390/agronomy10071019
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发表时间：
2020-07-01
期刊：
影响因子：
3.7
通讯作者：
Okazaki, Shin
中科院分区：
文献类型：
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作者：
Dastogeer, Khondoker Mohammad Golam;Oshita, Yutaro;Okazaki, Shin
为了了解自然栽培番茄植株组织中可培养真菌群落的分布情况，从日本3个地点采集的1895个茎组织中提取了1742个真菌分离物。总体而言，分离率很低(3-13%)，分离率和定殖率并不随品种的变化而显著变化。这些真菌被划分为29个独特的操作分类单元(OTUS)，其基因序列同源性为97%，其中大部分属于子囊菌门(99.3%)。可培养内生真菌的优势属为镰刀菌属(45.1%)、链格孢属(12.8%)、赤霉菌属(12.0%)和斑点落叶霉属(6.8%)。番茄内生真菌的α多样性因品种不同而不同。对不同品种的内生菌群落格局进行了排序分析，结果表明寄主品种对所有研究地点的内生菌群落组合都有显著影响。在所有品种上都发现了一些镰刀菌、交链孢属和青霉菌，但在不同品种中独有的很少。优势类群可能适应番茄茎中存在的特殊微生态和生理条件。
To understand the distribution of the cultivable fungal community in plant tissues from nature farming tomato plants, we sampled plants of seven different tomato cultivars and recovered 1742 fungal isolates from 1895 stem tissues sampled from three sites in Japan. Overall, the isolation frequency was low (3-13%) and the isolation and colonization frequencies did not vary significantly as a function of the cultivar. The fungi were divided into 29 unique operational taxonomic units (OTUs) with 97% ITS gene sequence identity, the majority of which belong to Ascomycota (99.3%). The dominant genera of cultivable endophytic fungi wereFusarium(45.1%),Alternaria(12.8%),Gibberella(12.0%), andDipodascus(6.8%). The alpha diversity of the fungal endophytes varied among tomato cultivars. Ordination analysis performed to investigate patterns of endophyte community assemblages on the various cultivars revealed that host cultivars had a significant impact on the endophyte community assemblages in all the study sites. Some of the taxaFusarium, Alternaria,andPenicilliumwere found on all cultivars, while few were uniquely present in different cultivars. The dominant taxa may be adapted to the particular microecological and physiological conditions present in tomato stems.