Cutting edge: B7/CD28 interactions regulate cell cycle progression independent of the strength of TCR signaling
Cutting edge: B7/CD28 interactions regulate cell cycle progression independent of the strength of TCR signaling
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DOI：
10.4049/jimmunol.169.12.6659
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发表时间：
2002-12-15
影响因子：
4.4
通讯作者：
Mueller, DL
中科院分区：
文献类型：
--
作者：
Bonnevier, JL;Mueller, DL
应用CFSE染料稀释法和流式细胞术检测B7/CD 28信号在Ag诱导的CD 4（+）T细胞周期进程中的作用。在野生型T细胞中，增殖与APC可用的Ag浓度直接相关。与此一致，G（0）-->G（1）细胞周期进展的速率随Ag浓度而变化。然而，T细胞母细胞的细胞分裂以恒定的速率发生，与Ag浓度无关。在中和性抗B7 mAb存在下培养的CD 28缺陷型CD 4（+）T细胞或野生型T细胞的G（0）->G（1）期进展减慢，证实在T细胞的初始活化中TCR和CD 28信号传导之间确实存在协同作用。然而，与TCR不同的是，CD 28刺激的强度也显示出在控制T细胞母细胞的细胞分裂速率方面发挥独特的作用。
The role of B7/CD28 signals in Ag-induced cell cycle progression of CD4(+) T cells was examined using the technique of CFSE dye dilution and flow cytometry. In wild-type T cells, proliferation was directly related to the concentration of Ag available to the APC. Consistent with this, the rate of G(0)-->G(1) cell cycle progression varied with the concentration of Ag. However, cell division by T cell blasts occurred at a constant rate, independent of Ag concentration. G(0)-->G(1) phase progression by CD28-deficient CD4(+) T cells or wild-type T cells cultured in the presence of neutralizing anti-B7 mAbs was slowed, confirming that a synergy does exist between TCR and CD28 signaling in the initial activation of the T cells. However, unlike the TCR, the strength of CD28 stimulation was also shown to play a unique role in controlling the rate of cell division by T cell blasts.