Regulation of Angiotensin II in Rat Adrenal Gland
Regulation of Angiotensin II in Rat Adrenal Gland
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血管紧张素II对大鼠肾上腺的调节
DOI：
10.1161/01.res.60.5.640
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发表时间：
1987
影响因子：
20.1
通讯作者：
F. Bumpus
中科院分区：
文献类型：
--
作者：
A. Husain;P. Desilva;R. Speth;F. Bumpus
大鼠肾上腺血管紧张素II免疫反应性水平比血浆高100倍以上。然而，目前尚不清楚肾上腺血管紧张素II免疫反应性的主要来源是局部肾素-血管紧张素系统的细胞内合成，还是血管紧张素II受体的摄取，还是两者兼而有之。我们的研究表明，肾上腺血管紧张素II的免疫反应性主要归因于血管紧张素II（>75%）。血管紧张素III（16%）和其他血管紧张素II片段也存在。肾上腺中大多数血管紧张素II免疫反应性（73%）、肾素活性（73%）和血管紧张素II受体结合活性（66%）位于包膜肾小球细胞层。2% NaCl渗吸引起的脱水降低了被囊-肾小球的这些活性。在肾上腺束状-髓质区域，脱水降低肾素活性，但不降低血管紧张素II免疫反应性或血管紧张素II受体结合活性。对照组和脱水大鼠的综合数据显示，荚膜-肾小球血管紧张素n免疫反应性与血管紧张素II受体结合活性密切相关（r =0.94, p <0.001），与肾素活性的相关性较弱，不显著（r = 0.66, p <0.1）。在束状-髓质细胞层中，血管紧张素II免疫反应性与肾素或血管紧张素n受体结合活性均无显著相关性。这些数据表明，大鼠肾上腺的不同功能层对血管紧张素II受体和肾素-血管紧张素系统的调节存在差异。这些数据还表明，肾上腺包膜-肾小球中血管紧张素II的大部分免疫反应性来自于受体介导的细胞外血管紧张素II的隔离，而不是由于细胞内肽的合成。
Levels of angiotensin II immunoreactivity In the rat adrenal gland are over one hundredfold higher than those in plasma. It is unclear, however, whether the major source of adrenal angiotensin II immunoreactivity is intracellular synthesis by a local renin-angiotensin system, uptake by angiotensin II receptors, or both. Our studies show that angiotensin II immunoreactivity in the adrenal gland is predominantly attributable to angiotensin II (>75%). Angiotensin III (16%) and other angiotensin II fragments are also present. The majority of angiotensin II immunoreactivity (73%), renin activity (73%), and angiotensin II receptor binding activity (66%) in the adrenal gland is located in the capsular glomerulosa cell layers. Dehydration produced by 2% NaCl imbibition decreased these activities in the capsular-glomerulosa. In the fasciculate-medullary regions of the adrenal gland, dehydration decreased renin activity but not angiotensin II immunoreactivity or angiotensin II receptor binding activity. Combined data from control and dehydrated rats showed a close correlation of the capsular-glomerulosa angiotensin n immunoreactivity with angiotensin II receptor binding activity (r =0.94, p <0.001) and a weaker, nonsignificant correlation with renin activity (r = 0.66, p <0.1). In the fasciculata-medullary cell layers, no significant correlations were found between angiotensin II immunoreactivity and either renin or angiotensin n receptor binding activity. These data demonstrate that functionally distinct layers of the rat adrenal gland differentially regulate angiotensin II receptors and the renin-angiotensin system. These data also suggest that the majority of angiotensin II immunoreactivity in the adrenal capsular-glomerulosa is derived from receptor-mediated sequestration of extracellular angiotensin II by its receptors and is not due to intracellular synthesis of the peptide.
肾上腺肾素-血管紧张素系统在调节钾刺激的醛固酮产生中的作用。
影响因子：
4.8
作者：
Nakamaru,M;Misono,KS;Naruse,M;Workman,RJ;Inagami,T
通讯作者：
Inagami,T
通过[3H]卡托普利放射自显影，血管紧张素转换酶定位于大鼠垂体和肾上腺。
影响因子：
4.8
作者：
Strittmatter,SM;DeSouza,EB;Lynch,DR;Snyder,SH
通讯作者：
Snyder,SH