Deletion of the autoregulatory insert modulates intraprotein electron transfer in rat neuronal nitric oxide synthase
Deletion of the autoregulatory insert modulates intraprotein electron transfer in rat neuronal nitric oxide synthase
复制标题
DOI：
10.1016/j.febslet.2008.07.005
复制
发表时间：
2008-08-06
期刊：
影响因子：
3.5
通讯作者：
Masters, Bettie Sue S.
中科院分区：
文献类型：
--
作者：
Feng, Changjian;Roman, Linda J.;Masters, Bettie Sue S.
对野生型和大鼠 nNOS 全酶自动调节 (AR) 插入缺失突变体进行 CO 光解动力学比较研究，以直接研究独特的 AR 插入在催化显着的 FMN-血红素蛋白内电子转移 (IET) 中的作用。尽管 IET 动力学轨迹的幅度降低了两到三倍，但 AR 缺失并没有改变钙调蛋白控制的 IET 的速率常数。这表明电子接受状态到新的电子供给（输出）状态的限速转换不涉及与AR插入物的相互作用，但AR一旦形成就可以稳定输出状态。 (c) 2008 年欧洲生化学会联合会。由 Elsevier B.V 出版。保留所有权利。
Comparative CO photolysis kinetics studies on wildtype and autoregulatory (AR) insert-deletion mutant of rat nNOS holoenzyme were conducted to directly investigate the role of the unique AR insert in the catalytically significant FMN-heme intraprotein electron transfer (IET). Although the amplitude of the IET kinetic traces was decreased two- to three-fold, the AR deletion did not change the rate constant for the calmodulin-controlled IET. This suggests that the rate-limiting conversion of the electron-accepting state to a new electron-donating ( output) state does not involve interactions with the AR insert, but that AR may stabilize the output state once it is formed. (c) 2008 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.