Visible spectrum extended-focus optical coherence microscopy for label-free sub-cellular tomography
Visible spectrum extended-focus optical coherence microscopy for label-free sub-cellular tomography
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DOI：
10.1364/boe.8.003343
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发表时间：
2017-07-01
影响因子：
3.4
通讯作者：
Lasser, Theo
中科院分区：
文献类型：
--
作者：
Marchand, Paul J.;Bouwens, Arno;Lasser, Theo
我们提出了一种新型的扩展焦光学相干显微镜（OCM），其轴向分辨率为0.7 μ m，横向分辨率为0.4 μ m，深度为40 μ m，同时保留了傅里叶域OCM的优点。我们的系统使用来自超连续激光源的超宽光谱。由于光谱范围从近红外到可见波长（带宽为240纳米），我们称该系统为visOCM。这种广谱与高na物镜的结合创造了几乎各向同性的3D亚微米分辨率。我们分析了visOCM在微珠样品上的成像性能，并证明了它在健康和阿尔茨海默病小鼠的细胞培养和离体脑组织上的成像质量。除了神经元细胞体、纤维和斑块外，脑组织的visOCM成像通过其高空间分辨率显示精细的血管结构和亚细胞特征。在活细胞中也观察到亚细胞结构，并通过传统用于OCT血管造影的方案进一步揭示。(C) 2017年美国光学学会
We present a novel extended-focus optical coherence microscope (OCM) attaining 0.7 mu m axial and 0.4 mu m lateral resolution maintained over a depth of 40 mu m, while preserving the advantages of Fourier domain OCM. Our system uses an ultra-broad spectrum from a supercontinuum laser source. As the spectrum spans from near-infrared to visible wavelengths (240 nm in bandwidth), we call the system visOCM. The combination of such a broad spectrum with a high-NA objective creates an almost isotropic 3D submicron resolution. We analyze the imaging performance of visOCM on microbead samples and demonstrate its image quality on cell cultures and ex-vivo brain tissue of both healthy and alzheimeric mice. In addition to neuronal cell bodies, fibers and plaques, visOCM imaging of brain tissue reveals fine vascular structures and sub-cellular features through its high spatial resolution. Sub-cellular structures were also observed in live cells and were further revealed through a protocol traditionally used for OCT angiography. (C) 2017 Optical Society of America