CHARACTERIZATION OF COCHLEAR OUTER HAIR CELL TURGOR
CHARACTERIZATION OF COCHLEAR OUTER HAIR CELL TURGOR
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DOI：
10.1152/ajpcell.1994.266.2.c467
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发表时间：
1994-02-01
影响因子：
--
通讯作者：
BROWNELL, WE
中科院分区：
文献类型：
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作者：
CHERTOFF, ME;BROWNELL, WE
耳蜗外毛细胞(OHC)是一种圆柱形细胞，其结构特征类似于液压骨架，即具有正内压的弹性壳。这项研究描述了OHC升高的胞浆压力在维持细胞形状中的作用。通过测量细胞形态随渗透压升高或降低的变化来估计豚鼠毛囊细胞内的压力。当渗透压持续增加时，细胞就会崩溃。崩溃发生在平均高于标准培养液8毫米柱的地方，这表明正常细胞的有效细胞内压力为128毫米汞柱。尽管灌注室中渗透压的最终变化与渗透压快速升高的细胞相比，在缓慢渗透压升高的情况下崩溃的细胞更少，但最终的渗透压变化是相似的。此外，细胞在没有渗透压变化或渗透压缓慢增加的情况下，体积会出现缓慢的自发增加，这表明细胞的内渗透压在体外增加。体积减小或升高后，细胞不会恢复到其初始体积。
The cochlear outer hair cell (OHC) is a cylindrical cell with structural features suggestive of a hydraulic skeleton, i.e., an elastic shell with a positive internal pressure. This study characterizes the role of the OHC elevated cytoplasmic pressure in maintaining the cell shape. Intracellular pressure of OHCs from guinea pig is estimated by measuring changes in cell morphology in response to increasing or decreasing osmolarity. Cells collapse when subjected to a continuous increase in osmolarity. Collapse occurs at an average of 8 mosM above the standard medium, suggesting that normal cells have an effective intracellular pressure of 128 mmHg. Fewer cells collapse when exposed to slow rates of osmolarity increase than cells exposed to fast rates of osmolarity increase, although the final change in osmolarity in the perfusion chamber is similar. Furthermore, cells undergo a slow, spontaneous increase in volume on exposure to either no osmolarity change or slow rates of osmolarity increase, suggesting that the cell's internal osmolarity increases in vitro. After volume reduction or elevation, cells do not return to their initial volume.