Histopathologic time course of myocardial infarct in rabbit hearts
Histopathologic time course of myocardial infarct in rabbit hearts
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DOI：
10.1016/s1054-8807(02)00115-1
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发表时间：
2002-11-01
影响因子：
3.7
通讯作者：
Gelpi, RJ
中科院分区：
文献类型：
--
作者：
Morales, C;González, GE;Gelpi, RJ
前言：研究了兔心肌梗死及远隔带的组织病理学演变。方法：结扎55只兔左冠状动脉，分别于结扎后2、4、6、8、12、14、16、185、26、35、56d处死动物，每组5只。对照组2只，假手术组4只。切取心脏，切片，苏木精三色、马森氏三色和天狼星红染色。组织学评价为半定量(评分0~+)。结果：缺血后第2天，中性粒细胞呈++状态，第4天突然减少，第6天完全消失，第4~14天以成纤维细胞为特征的细胞增殖增加。中层心肌凝固性坏死，第1周为++。心肌梗死后第2天至56天有明显的心内膜下肌细胞溶解。在第二周，观察到淋巴细胞和巨噬细胞的增殖(++)，肉芽组织的形成(++)和早期的纤维化痕迹，在第35天达到高峰。在第56天(++)，瘢痕形成完全。在远隔区(右室和右室间隔)，Vimentin上的细胞在第2天开始增殖，血管周围、间质和心内膜纤维化在第6天开始增加，在第16天达到高峰。结论：虽然兔心肌梗死保持了梗死时间的本质，但在梗死区Vimentin上细胞+的早期出现和心内膜下纤维化，以及中性粒细胞的快速增加和早期消失与其他物种不同。一个有趣的发现是细胞的早期增殖，在偏远地区具有成纤维细胞的特征。(C)2002 Elsevier Science Inc.保留所有权利。
Introduction: The histopathologic evolution of myocardial infarct and of remote zones in rabbit hearts was studied. Methods: The left coronary artery of 55 rabbits was ligated and rabbits were sacrificed at 2, 4, 6, 8, 12, 14, 16, 185 26, 35 and 56 days post-ligature (n = 5 per group). Two rabbits were used as control and four were sham-operated. The hearts were excised, cut in slices and stained with hematoxylineosin, Masson's trichrome and picrosirius red. The histological evaluation was semiquantitative (scale: 0 to ++). Results: At day 2, the presence of neutrophils was ++, decreasing suddenly at day 4 and disappearing completely at day 6. The proliferation of cells with features of fibroblasts increased from days 4 to 14 post-occlusion. Coagulation necrosis in mid-myocardium during the first week was ++. Subendocardial myocytolysis was evident from day 2 up to day 56 post-infarction. During the second week, proliferation of lymphocytes and macrophages (++), granulation tissue formation (++) and incipient traces of fibrosis that peaked at day 35 were observed. Scarring was complete at day 56 (++). In remote zones (right ventricle and septum), the proliferation of cells+ on Vimentin was observed at day 2, and perivascular, interstitial and endocardial fibrosis started to increase at day 6 and peaked at day 16. Conclusion: Although myocardial infarction in rabbits maintains the essence of the infarct chronology, some differences as the early presence of cells+ on Vimentin and subendocardial fibrosis in infarcted areas, and also the rapid increase and early disappearance of neutrophils appear when other species are considered. An interesting finding was the early proliferation of cells with features of fibroblasts in remote zones. (C) 2002 Elsevier Science Inc. All rights reserved.