Food Compounds Activating Thermosensitive TRP Channels in Asian Herbal and Medicinal Foods.
Food Compounds Activating Thermosensitive TRP Channels in Asian Herbal and Medicinal Foods.
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DOI：
10.3177/jnsv.61.s86
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发表时间：
2015-05
影响因子：
1.6
通讯作者：
Tatsuo Watanabe;Y. Terada
中科院分区：
文献类型：
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作者：
Tatsuo Watanabe;Y. Terada
热敏瞬时受体电位（TRP）离子通道包括辣椒素受体TRPV1。激活TRPV1的食物成分通过刺激交感神经抑制体脂沉积。TRPA1是对刺激性化合物的另一种刺激性传感器，主要与TRPV1在感觉神经末梢共表达。因此，TRPA1激活有望具有与TRPV1激活相似的抗肥胖作用。我们利用表达TRPV1和TRPA1的细胞从亚洲香料中寻找TRPV1和TRPA1的激动剂。此外，我们对小鼠进行了高脂肪和高糖饮食的食物成分添加测试。我们发现辣椒和甜椒中的辣椒素、辣椒碱、辣椒素、黑胡椒中的胡椒碱类似物、生姜中的姜辣素和姜辣素、日本辣椒中的三酚和羟基三酚都是TRPV1激动剂。我们还从大蒜和榴莲中鉴定出几种硫化物，从蜂王浆中鉴定出羟基脂肪酸，从豆蔻（生姜）中鉴定出miogadial和miogatrial，从黑胡椒中鉴定出胡椒碱类似物，从高良姜（Alpinia galanga）中鉴定出醋酸乙氧基chavicol （ACA）作为TRPA1激动剂。饲粮中添加胡椒碱可减少内脏脂肪，提高肩胛间棕色脂肪组织（IBAT）中的解偶联蛋白1 (UCP1)，其中黑胡椒提取物的作用强于胡椒碱。肉桂醛和ACA作为TRPA1激动剂抑制脂肪沉积，增加UCP1。我们发现几种TRPV1和TRPA1激动剂以及一些TRPV1和TRPA1激动剂抑制小鼠内脏脂肪沉积。这些化合物对人体的影响还有待进一步研究，但我们预计它们将有助于预防肥胖。
There are several thermosensitive transient receptor potential (TRP) ion channels including capsaicin receptor, TRPV1. Food components activating TRPV1 inhibit body fat deposition through sympathetic nerve stimulation. TRPA1 is another pungency sensor for pungent compounds and is mainly coexpressed with TRPV1 in sensory nerve endings. Therefore, TRPA1 activation is expected to have an anti-obesity effect similar to TRPV1 activation. We have searched for agonists for TRPV1 and TRPA1 in vitro from Asian spices by the use of TRPV1- and TRPA1-expressing cells. Further, we performed food component addition tests to high-fat and high-sucrose diets in mice. We found capsiate, capsiconiate, capsainol from hot and sweet peppers, several piperine analogs from black pepper, gingeriols and shogaols from ginger, and sanshools and hydroxysanshools from sansho (Japanese pepper) to be TRPV1 agonists. We also identified several sulfides from garlic and durian, hydroxy fatty acids from royal jelly, miogadial and miogatrial from mioga (Zingiber mioga), piperine analogs from black pepper, and acetoxychavicol acetate (ACA) from galangal (Alpinia galanga) as TRPA1 agonists. Piperine addition to diets diminished visceral fats and increased the uncoupling protein 1 (UCP1) in interscapular brown adipose tissue (IBAT), and black pepper extract showed stronger effects than piperine. Cinnamaldehyde and ACA as TRPA1 agonists inhibited fat deposition and increased UCP1. We found that several agonists of TRPV1 and TRPA1 and some agonists of TRPV1 and TRPA1 inhibit visceral fat deposition in mice. The effects of such compounds on humans remain to be clarified, but we expect that they will be helpful in the prevention of obesity.