A Study of the Vaginal Microbiome in Healthy Canadian Women Utilizing cpn60-Based Molecular Profiling Reveals Distinct Gardnerella Subgroup Community State Types
A Study of the Vaginal Microbiome in Healthy Canadian Women Utilizing cpn60-Based Molecular Profiling Reveals Distinct Gardnerella Subgroup Community State Types
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DOI：
10.1371/journal.pone.0135620
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发表时间：
2015-08-12
期刊：
影响因子：
3.7
通讯作者：
Money, Deborah
中科院分区：
文献类型：
--
作者：
Albert, Arianne Y. K.;Chaban, Bonnie;Money, Deborah
阴道微生物群对于女性的生殖和整体健康很重要。然而，这种复杂微生物群落的结构、功能和动态与健康结果之间的关系仍然难以捉摸。本研究的目的是确定健康、非怀孕、种族多样化、育龄加拿大女性阴道微生物群中原核生物的系统发育范围和丰度。收集了 310 名女性的社会人口学、行为和临床数据，并对阴道拭子进行了分析。通过 Chaperonin-60 通用靶标的焦磷酸测序生成了其阴道微生物组的详细概况。通过层次聚类划分了六种社区状态类型 (CST)，包括三种以乳杆菌为主的 CST（卷曲乳杆菌、L. iners、L. jensenii）、两种以加德纳菌为主的 CST（A 和 C 亚组）和一种“中间”CST，其中包括少数女性，其微生物群由其他七种物种主导，或没有优势物种，但有少数链球菌、葡萄球菌、共显性群落中的嗜胃杆菌、大肠杆菌和各种变形菌。 Nugent 评分和深度测序 CST 之间惊人的对应关系继续强化了更简单的革兰氏染色方法提供的基本前提，而其他分析揭示了基于 cpn60 的详细系统发育和阴道样本中微生物群落的估计丰度。种族是预测 CST 的唯一人口统计学或临床特征，亚洲和白人女性之间存在差异（p = 0.05）。总之，这项研究证实了之前描述加德纳菌四个基于 cpn60 的亚群的工作，揭示了之前未描述的 CST。这些数据描述了在没有特定阴道健康问题的加拿大女性中观察到的细菌群落范围，并为未来对临床重要群体的研究提供了重要的基线。
The vaginal microbiota is important in women's reproductive and overall health. However, the relationships between the structure, function and dynamics of this complex microbial community and health outcomes remain elusive. The objective of this study was to determine the phylogenetic range and abundance of prokaryotes in the vaginal microbiota of healthy, non-pregnant, ethnically diverse, reproductive-aged Canadian women. Sociodemographic, behavioural and clinical data were collected and vaginal swabs were analyzed from 310 women. Detailed profiles of their vaginal microbiomes were generated by pyrosequencing of the chaperonin-60 universal target. Six community state types (CST) were delineated by hierarchical clustering, including three Lactobacillus-dominated CST (L. crispatus, L. iners, L. jensenii), two Gardnerella-dominated (subgroups A and C) and an "intermediate" CST which included a small number of women with microbiomes dominated by seven other species or with no dominant species but minority populations of Streptococcus, Staphylococcus, Peptoniphilus, E. coli and various Proteobacteria in co-dominant communities. The striking correspondence between Nugent score and deep sequencing CST continues to reinforce the basic premise provided by the simpler Gram stain method, while additional analyses reveal detailed cpn60-based phylogeny and estimated abundance in microbial communities from vaginal samples. Ethnicity was the only demographic or clinical characteristic predicting CST, with differences in Asian and White women p = 0.05). In conclusion, this study confirms previous work describing four cpn60-based subgroups of Gardnerella, revealing previously undescribed CST. The data describe the range of bacterial communities seen in Canadian women presenting with no specific vaginal health concerns, and provides an important baseline for future investigations of clinically important cohorts.