Assessing the viability of mutant and manipulated sperm by artificial insemination of Caenorhabditis elegans.
Assessing the viability of mutant and manipulated sperm by artificial insemination of Caenorhabditis elegans.
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通过秀丽隐杆线虫人工授精评估突变和操纵精子的活力。
DOI：
10.1093/genetics/138.3.689
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发表时间：
1994
期刊：
影响因子：
3.3
通讯作者：
Ward,S
中科院分区：
文献类型：
--
作者：
LaMunyon,CW;Ward,S
我们描述了一个方案的人工授精秀丽隐杆线虫，我们用来评估精子的活力，从不同的菌株和精子在体外激活。蠕虫可以人工授精，几乎100%成功。雄性和雌雄同体的精子都可以用于人工授精。从一个不育雌雄同体[fem3 (q23ts)]中发现精子是可存活的。就像正常的交配一样，雄性精子通过人工授精形成雌雄同体，从而在竞争中战胜雌雄同体自身的精子，即使它们没有被精液射精。用弱碱三乙醇胺对精子进行体外活化，精子可存活。相比之下，体外蛋白酶处理的精子没有被激活。
We describe a protocol for artificial insemination of Caenorhabditis elegans which we used to evaluate the viability of sperm from different strains and of sperm activated in vitro. Worms can be artificially inseminated with almost 100% success. Both male and hermaphrodite sperm can be used for insemination. Sperm from a sterile hermaphrodite [fem-3(q23ts)] were found to be viable. As with normal mating, male sperm inseminated into hermaphrodites artificially outcompete the hermaphrodite's own sperm, even though they have not been ejaculated with seminal fluid. Spermatozoa that were activated in vitro from spermatids by the weak base triethanolamine were viable. In contrast, spermatozoa activated in vitro by protease treatment were not.