Probing the flavivirus membrane fusion mechanism by using monoclonal antibodies
Probing the flavivirus membrane fusion mechanism by using monoclonal antibodies
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DOI：
10.1128/jvi.01041-07
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发表时间：
2007-10-01
影响因子：
5.4
通讯作者：
Heinz, Franz X.
中科院分区：
文献类型：
--
作者：
Stiasny, Karin;Brandler, Samantha;Heinz, Franz X.
在这项研究中，我们研究了在黄病毒模型（蜱传脑炎病毒）的融合抑制机制的单克隆抗体针对不同的结构域的融合蛋白（E）和不同的网站内的每个域通过使用体外融合试验。我们的数据表明，根据其结合位点的位置，单克隆抗体损害融合过程的早期或晚期，通过阻断与靶膜的初始相互作用或通过干扰E的融合后结构的正确形成。这些数据提供了新的见解黄病毒融合抑制抗体的机制和它们可能的贡献病毒中和。
In this study, we investigated in a flavivirus model (tick-borne encephalitis virus) the mechanisms of fusion inhibition by monoclonal antibodies directed to the different domains of the fusion protein (E) and to different sites within each of the domains by using in vitro fusion assays. Our data indicate that, depending on the location of their binding sites, the monoclonal antibodies impaired early or late stages of the fusion process, by blocking the initial interaction with the target membrane or by interfering with the proper formation of the postfusion structure of E, respectively. These data provide new insights into the mechanisms of flavivirus fusion inhibition by antibodies and their possible contribution to virus neutralization.