Cellular heterogeneity in the production of an anti-hapten antibody.
Cellular heterogeneity in the production of an anti-hapten antibody.
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DOI：
10.1084/jem.126.1.159
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发表时间：
1967-07-01
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影响因子：
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通讯作者：
Mäkelä O
中科院分区：
文献类型：
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作者：
Mäkelä O
用3-碘-4-羟基-5-硝基苯基乙酸氯（NIP）和鸡球蛋白的缀合物免疫大鼠，检测其单个淋巴结细胞对NIP或NNP与噬菌体缀合的抗体。NNP与NIP相关，但具有硝基而不是碘。缀合的半抗原仍具有感染性，但在10-7的血清浓度下容易被抗半抗原血清灭活。携带相关半抗原的噬菌体对抗体的敏感性低于与免疫原性半抗原缀合的噬菌体。当将NIP-T2和NNP-T4缀合物在含有来自抗NIP动物的单个淋巴结细胞的微滴中孵育时，在某些情况下发生任一种或两种NIP-T4的强烈失活。由于文中给出的原因，认为该灭活是由于微滴中细胞产生的抗NIP抗体所致。抗NIP细胞对这两种细胞的灭活是高度非随机的;一些细胞几乎灭活了液滴中大约1700个NIP-T2细胞，但几乎所有的NNP-T4细胞都是活的。其他人有一个非常强的交叉反应和灭活相等比例（高达75%）的两种酶。剩余的活性细胞表现出中等程度的交叉反应。对照实验表明，不同细胞产生的抗NIP的特异性存在真正的细胞异质性。他们认为观察到的特异性异质性仅限于半抗原的结构，不受载体结构的影响。由于研究的所有细胞均由单一半抗原诱导，因此观察到的细胞异质性可能不是由异质性抗原引起的。似乎更可能的是，在细胞被NIP半抗原刺激之前，它们中存在潜在的异质性。
Single lymph node cells from rats immunized with a conjugate of 3-iodo-4-hydroxy-5-nitrophenyl acetic acid chloride (NIP) and chicken globulin were tested against either NIP or NNP conjugated with bacteriophage. NNP is related to NIP but has a nitro group instead of iodine. The conjugated phages were still infective but easily inactivated by anti-hapten sera at a serum concentration of 10–7. Phages carrying a related hapten were less sensitive to antibody than phages conjugated with the immunogenic hapten. When NIP-T2 and NNP-T4 conjugates were incubated in microdrops containing a single lymph node cell from an anti-NIP animal, strong inactivation of either or both phages occurred in some cases. For the reasons given in the text this inactivation was considered to be due to anti-NIP antibody produced by the cell in the microdrop. Inactivation of the two kinds of phages by the anti-NIP cells was highly nonrandom; some cells inactivated almost all of the 1700 or so NIP-T2 phages in the drop but left almost all NNP-T4 phages viable. Others had a very strong cross-reaction and inactivated equal proportions (up to 75%) of both phages. The remaining active cells exhibited an intermediate degree of cross-reaction. Control experiments indicated that there was a true cellular heterogeneity in the specificity of the anti-NIP produced by different cells. They suggested that the observed heterogeneity of specificities was limited to the structure of the hapten and was not influenced by the structure of the carrier. As all the cells studied were induced by a single hapten, the observed cellular heterogeneity was probably not caused by a heterogeneous antigen. It seems more likely that latent heterogeneity was present in the cells before they were stimulated by the NIP hapten.