Comparative structural and techno-functional elucidation of full-fat and defatted flaxseed extracts: implication of atmospheric pressure plasma jet
Comparative structural and techno-functional elucidation of full-fat and defatted flaxseed extracts: implication of atmospheric pressure plasma jet
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全脂和脱脂亚麻籽提取物的比较结构和技术功能阐明：大气压等离子体射流的意义
DOI：
10.1002/jsfa.11418
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发表时间：
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影响因子：
4.1
通讯作者：
Geng Fang
中科院分区：
文献类型：
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作者：
Nie Chengzhen;Qin Xiaopeng;Duan Ziqiang;Huang Shasha;Yu Xiao;Deng Qianchun;Xiang Qisen;Geng Fang
亚麻籽蛋白/多糖复合物的技术功能相对较差，特别是在乳化和抗氧化活性方面，部分限制了它们在健康食品系统中的应用。本研究旨在研究大气压等离子体射流（APPJ）对亚麻籽提取物的理化、结构和选定技术功能特性的影响。结果表明，全脂和脱脂亚麻籽提取物溶液（5 mg mL-1）显示pH持续下降（− 54.06%，− 48.80%，P < 0.05）和zeta电位值（-29.42%，-44.28%，P < 0.05），但在APPJ处理0-120 s期间，通过光学显微镜观察到颗粒尺寸逐渐增加。此外，APPJ导致最初的减少，但随后增加的蛋白质羰基和二次脂质氧化产物，并同时改变了亚麻籽提取物的空间构象和微观结构，如内源性荧光特性和扫描电子显微镜（SEM）所示。此外，暴露于APPJ 30 s后，全脂和脱脂亚麻籽提取物的蛋白质亚基重塑和树胶多糖解聚不同。重要的是，脱脂亚麻籽提取物的乳化和抗氧化活性得到了特别的改善，如通过在APPJ处理15-30秒后的cyro-SEM和2，2-二苯基-1-苦基肼基自由基清除活性所评估的，这是由于蛋白质和树胶多糖之间的相互作用发生了变化，以及特定酚类化合物的释放。结论APPJ可以作为一个有前途的战略，定制特定的技术功能的亚麻籽基于温和结构修饰的提取物。© 2021化学工业协会。
BACKGROUNDThe relatively inferior techno‐functionality of flaxseed protein/polysaccharide complexes, especially regarding emulsifying and antioxidant activities, has partially limited their implication in the health food system. The present study aimed to investigate the effects of an atmospheric pressure plasma jet (APPJ) on the physicochemical, structural and selected techno‐functional properties of flaxseed extracts.RESULTSThe results obtained showed that the full‐fat and defatted flaxseed extract solutions (5 mg mL–1) displayed a sustainable decline in pH (−54.06%, −48.80%,P< 0.05) and zeta potential values (−29.42%, −44.28%,P< 0.05), but a gradual increase in particle sizes, as visualised by an optical microscope, during 0–120 s of APPJ treatment. Moreover, the APPJ led to initial decrease but subsequent increase in protein carbonyls and secondary lipid oxidation products, and concurrently changed the spatial conformation and microstructure of flaxseed extracts, as indicated by endogenous fluorescence properties and scanning electron microscopy (SEM). Additionally, the protein subunit remodeling and gum polysaccharides depolymerization were different for full‐fat and defatted flaxseed extracts after 30 s of APPJ exposure. Importantly, the emulsifying and antioxidant activities of defatted flaxseed extract were particularly improved, as assessed by cyro‐SEM and 2,2‐diphenyl‐1‐picrylhydrazyl radical scavenging activity following 15–30 s of APPJ treatment, as a result of the changing interactions between protein and gum polysaccharides, as well as the release of specific phenolic compounds.CONCLUSIONAPPJ could serve as a promising strategy for tailoring the specific techno‐functionality of flaxseed extracts based on mild structural modification. © 2021 Society of Chemical Industry.