Mercapturic acids as metabolites of alkylating substances in urine samples of German inhabitants
Mercapturic acids as metabolites of alkylating substances in urine samples of German inhabitants
复制标题
DOI：
10.1016/j.ijheh.2011.03.001
复制
发表时间：
2011-06-01
影响因子：
6
通讯作者：
Goeen, Thomas
中科院分区：
文献类型：
--
作者：
Eckert, Elisabeth;Schmid, Klaus;Goeen, Thomas
羟基烷基硫代尿酸(HAMA)是丙烯醛、1，3-丁二烯、环氧乙烷、环氧丙烷、缩水甘油等具有致癌潜力的烷基化物质的主要尿代谢物。这些烷基化物质在工业过程中被广泛使用，但它们也存在于环境中，例如烟草烟雾中。这项研究的目的是测定吸烟者和非吸烟者尿液中作为暴露生物标志物的六种HAMA。我们应用了一种灵敏的分析方法，使用亲水相互作用液相色谱-串联质谱仪(HILIC-MS/MS)来测定环氧乙烷的生物标记物2-羟乙基硫代尿酸(HEMA)、环氧丙烷的生物标记物2-羟丙基硫代尿酸(2-HPMA)、丙烯醛的生物标记物3-羟丙基硫代尿酸(3-HPMA)、2，3-二羟丙基硫代尿酸(DHPMA，缩水甘油的生物标记物)以及3，4-二羟基丁基和3-单羟基丁烯硫代尿酸(DHBMA和MHBMA，1，3-丁二烯的生物标记物)。在我们分析的每个尿样中检测到四种HAMA的本底浓度。硫代尿酸HEMA和MHBMA的检出率分别为55%和10%。在非吸烟者(n=54)的尿液中，我们观察到的中位数水平分别为206，1.6，12.1,146,159和
Hydroxyalkyl mercapturic acids (HAMA) are the main urinary metabolites of several alkylating substances that possess a carcinogenic potential, like acrolein, 1,3-butadiene, ethylene oxide, propylene oxide and glycidol. These alkylating substances are used extensively in industrial processes, but they do also occur environmentally, e.g. in tobacco smoke. The aim of this study was the determination of six HAMA, as biomarkers of exposure, in human urine of smokers and non-smokers. We applied a sensitive analytical method, using hydrophilic interaction liquid chromatography with tandem mass spectrometry (HILIC-MS/MS) for the determination of 2-hydroxyethyl mercapturic acid (HEMA, biomarker for ethylene oxide), 2-hydroxypropyl mercapturic acid (2-HPMA, biomarker for propylene oxide), 3-hydroxypropyl mercapturic acid (3-HPMA, biomarker for acrolein), 2,3-dihydroxypropyl mercapturic acid (DHPMA, biomarker for glycidol) as well as 3,4-dihydroxybutyl mercapturic acid and 3-monohydroxybutenyl mercapturic acids (DHBMA and MHBMA, biomarkers for 1,3-butadiene). Background concentrations of four HAMA were detected in each urine sample we analyzed. The mercapturic acids HEMA and MHBMA were detected in 55% and 10% of the samples, respectively. In the urine of non-smokers (n = 54) we observed median levels of 206, 1.6, 12.1, 146, 159, and