Phylogenetic Analysis of Chlamydia trachomatis Tarp and Correlation with Clinical Phenotype
Phylogenetic Analysis of Chlamydia trachomatis Tarp and Correlation with Clinical Phenotype
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DOI：
10.1128/iai.00515-10
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发表时间：
2010-09-01
影响因子：
3.1
通讯作者：
Hackstadt, Ted
中科院分区：
文献类型：
--
作者：
Lutter, Erika I.;Bonner, Christine;Hackstadt, Ted
沙眼衣原体是全世界感染性失明的主要原因，也是引起性传播感染的最常见的病原体。TARP(易位肌动蛋白募集磷蛋白)是沙眼衣原体感染的中心环节，是一种介导肌动蛋白成核的III型分泌型效应器。对参考毒株以及眼部、生殖器和淋巴肉芽肿(LGV)临床分离株的TARP系统发育分析表明，TARP与疾病表型之间存在进化关系，LGV和眼部分离株与泌尿生殖道分离株分成不同的分支。TARP的序列分析表明，在临床分组中具有高度的变异性和确定的趋势。LGV毒株的Tarp含有最多的富含酪氨酸的重复区域(最多9个)和最少的(2个)预测的肌动蛋白结合结构域。相反，来自眼部分离株的Tarp蛋白包含多达四个肌动蛋白结合域和少至一个富含酪氨酸的重复区域。结果表明，在沙眼衣原体适应宿主内特定生态位的过程中，Tarp基因是为数不多的几个已知基因之一。
Chlamydia trachomatis is the leading cause of infectious blindness worldwide and is the most commonly reported pathogen causing sexually transmitted infections. Tarp (translocated actin recruiting phosphoprotein), a type III secreted effector that mediates actin nucleation, is central to C. trachomatis infection. The phylogenetic analysis of tarP from reference strains as well as ocular, genital, and lymphogranuloma venereum (LGV) clinical isolates demonstrated an evolutionary relationship with disease phenotype, with LGV and ocular isolates branched into clades that were separate from the urogenital isolates. The sequence analysis of Tarp indicated a high degree of variability and identified trends within clinical groupings. Tarps from LGV strains contained the highest number of tyrosine-rich repeat regions (up to nine) and the fewest (two) predicted actin binding domains. The converse was noted for Tarp proteins from ocular isolates that contained up to four actin binding domains and as few as one tyrosine-rich repeat region. The results suggest that Tarp is among the few known genes to play a role in C. trachomatis adaptations to specific niches within the host.