A normative study of the synovial fluid proteome from healthy porcine knee joints.
A normative study of the synovial fluid proteome from healthy porcine knee joints.
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DOI：
10.1021/pr500587x
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发表时间：
2014-10-03
影响因子：
4.4
通讯作者：
Steen, Hanno
中科院分区：
文献类型：
--
作者：
Bennike, Tue;Ayturk, Ugur;Haslauer, Carla M.;Froehlich, John W.;Proffen, Benedikt L.;Barnaby, Omar;Birkelund, Svend;Murray, Martha M.;Warman, Matthew L.;Stensballe, Allan;Steen, Hanno
关节中的滑液含有来源于血浆的蛋白质和由关节组织（例如滑膜、软骨、韧带和半月板）内的细胞产生的蛋白质。健康滑液的蛋白质组组成和许多滑液组分的细胞起源尚未完全了解。在这里，我们提出了一个规范的蛋白质组学研究使用猪滑液。使用我们优化的方法，我们在健康滑液中鉴定了267种具有高置信度的蛋白质。我们还评估了来自组织的mRNA表达数据，这些组织可以有助于滑液蛋白质组，包括滑膜，软骨，血液和肝脏，以更好地估计这些来源对特定滑液组分的相对贡献。我们在健康滑液中鉴定了113种蛋白质，这些蛋白质似乎主要来源于血浆渗出液，37种蛋白质主要来源于滑膜，11种蛋白质主要来源于软骨。最后，我们比较了确定的滑液蛋白质组的人血浆的蛋白质组，我们发现，这两种体液有许多相似之处，强调了许多滑液成分的检测血浆衍生的性质。了解健康关节的滑液蛋白质组将有助于确定导致关节疾病的机制和疾病进展的途径。
Synovial fluid in an articulating joint contains proteins derived from the blood plasma and proteins that are produced by cells within the joint tissues, such as synovium, cartilage, ligament, and meniscus. The proteome composition of healthy synovial fluid and the cellular origins of many synovial fluid components are not fully understood. Here, we present a normative proteomics study using porcine synovial fluid. Using our optimized method, we identified 267 proteins with high confidence in healthy synovial fluid. We also evaluated mRNA expression data from tissues that can contribute to the synovial fluid proteome, including synovium, cartilage, blood, and liver, to better estimate the relative contributions from these sources to specific synovial fluid components. We identified 113 proteins in healthy synovial fluid that appear to be primarily derived from plasma transudates, 37 proteins primarily derived from synovium, and 11 proteins primarily derived from cartilage. Finally, we compared the identified synovial fluid proteome to the proteome of human plasma, and we found that the two body fluids share many similarities, underlining the detected plasma derived nature of many synovial fluid components. Knowing the synovial fluid proteome of a healthy joint will help to identify mechanisms that cause joint disease and pathways involved in disease progression.
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DOI：
10.1073/pnas.79.22.6861
发表时间：
1982-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
BRESLOW, JL;ROSS, D;ZANNIS, VI
通讯作者：
ZANNIS, VI
影响因子：
--
作者：
Lawrence, Reva C.;Felson, David T.;Wolfe, Frederick
通讯作者：
Wolfe, Frederick
影响因子：
4.3
作者：
Bennike, Tue;Birkelund, Svend;Andersen, Vibeke
通讯作者：
Andersen, Vibeke
影响因子：
2.6
作者：
Desrosiers, Richard R.;Beaulieu, Edith;Beliveau, Richard
通讯作者：
Beliveau, Richard
影响因子：
3.4
作者：
Kollipara, Laxmikanth;Zahedi, Rene P.
通讯作者：
Zahedi, Rene P.