MPF components and meiotic competence in growing pig oocytes
MPF components and meiotic competence in growing pig oocytes
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猪生长卵母细胞的 MPF 成分和减数分裂能力
DOI：
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发表时间：
1994
影响因子：
2.5
通讯作者：
R. Moor
中科院分区：
文献类型：
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作者：
Leandro Christmann;Thomas Jung;R. Moor
生长期猪卵母细胞（直径≤90 μm）在体外不能恢复减数分裂。我们目前实验的目的是确定这些细胞减数分裂能力的原因。通过比较组蛋白H1激酶活性在生长和完全生长的卵母细胞，我们证明，无能与无法激活H1激酶在生长的卵母细胞。免疫印迹法用于确定这种激酶失活是否是由于缺乏p34 cdc 2蛋白或B-型细胞周期蛋白所致。结果表明，这些细胞周期分子中的每一种在生长和完全生长的卵母细胞中都以相当的量存在。在第三系列的研究中，进行了实验，试图在生长过程中诱导p34 cdc 2激活。冈田酸是一种磷酸酶1和2A的抑制剂，已知在许多不同物种中刺激和加速减数分裂周期向M期的转变，用冈田酸处理能够诱导p34 cdc 2激酶活性，并促进生长中卵母细胞的G2期至M期。
Growing pig oocytes (≤90 μm in diameter) are unable to resume meiosis in vitro. The objective of our present experiments has been to identify the reasons for meiotic competence in these cells. By comparing histone H1 kinase activity in growing and fully grown oocytes we demonstrate that incompetence is associated with an inability to activate H1 kinase in growing oocytes. Immunoblotting was used to determine whether this kinase inactivity resulted from a lack of either p34cdc2 protein or B‐type cyclin. The results established that each of these cell cycle molecules are present in comparable amounts in both growing and fully grown oocytes. In the third series of studies experiments were carried out in an attempt to induce p34cdc2 activation during growth. Treatment with okadaic acid, an inhibitor of phosphatase 1 and 2A known to stimulate and accelerate the transition into M‐phase of the meiotic cycle in a number of different species, was able to induce p34cdc2 kinase activity and facilitated the G2‐ to M‐phase in growing oocytes.