Three-Dimensional Imaging Analysis for Skeletal Muscle.
Three-Dimensional Imaging Analysis for Skeletal Muscle.
复制标题
骨骼肌三维成像分析。
DOI：
10.1007/978-1-0716-3036-5_32
复制
发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Asakura,Atsushi
中科院分区：
文献类型：
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作者：
Karthikeyan,Smrithi;Kim,Kyutae;Asakura,Yoko;Verma,Mayank;Asakura,Atsushi
骨骼肌是一种高度有序的组织，由多种细胞组成的复杂网络组成。在动态平衡和损伤期间，这些细胞之间的动态空间和时间相互作用使骨骼肌具有再生能力。为了正确理解再生过程，必须进行三维(3-D)成像过程。虽然已经有几个研究3D成像的方案，但它主要集中在神经系统上。该协议旨在概述使用来自共聚焦显微镜图像的空间数据来呈现骨骼肌的3-D图像的工作流程。该协议使用ImageJ、Ilastik和Imaris软件进行3-D渲染和计算图像分析，因为这两种软件都相对容易使用，并且具有强大的分割功能。
Skeletal muscle is a highly ordered tissue composed of a complex network of a diverse variety of cells. The dynamic spatial and temporal interaction between these cells during homeostasis and during times of injury gives the skeletal muscle its regenerative capacity. In order to properly understand the process of regeneration, a three-dimensional (3-D) imaging process must be conducted. While there have been several protocols studying 3-D imaging, it has primarily been focused on the nervous system. This protocol aims to outline the workflow for rendering a 3-D image of the skeletal muscle using spatial data from confocal microscope images. This protocol uses the ImageJ, Ilastik, and Imaris software for 3-D rendering and computational image analysis as both are relatively easy to use and have powerful segmentation capabilities.