COPII coat assembly and selective export from the endoplasmic reticulum.
COPII coat assembly and selective export from the endoplasmic reticulum.
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DOI：
10.1093/jb/mvh184
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发表时间：
2004-12
影响因子：
2.7
通讯作者：
Ken Sato
中科院分区：
文献类型：
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作者：
Ken Sato
外壳蛋白复合物II（COPII）产生转运囊泡，介导蛋白质从内质网（ER）转运。最近的结构和生物化学研究表明，COPII涂层负责直接捕获膜货物蛋白和ER膜的物理变形，驱动运输囊泡的形成。ER膜上COPII包被囊泡的形成是通过GDP/GTP交换激活Ras样小GTP酶Sar 1而触发的，而激活的Sar 1反过来又促进COPII包被组装。随后的GTP水解的Sar 1导致解体的外壳蛋白，然后再循环的额外轮的囊泡形成。因此，认为Sar 1 GT3循环调节COPII涂层组装和拆卸。新出现的证据表明，货物蛋白调节Sar 1 GTP水解，以协调涂层组装与货物选择。在这里，我讨论了可能的作用的GTP水解的Sar 1在COPII外套组装和选择性摄取货物蛋白进入运输囊泡。
The coat protein complex II (COPII) generates transport vesicles that mediate protein transport from the endoplasmic reticulum (ER). Recent structural and biochemical studies have suggested that the COPII coat is responsible for direct capture of membrane cargo proteins and for the physical deformation of the ER membrane that drives the transport vesicle formation. The COPII-coated vesicle formation at the ER membrane is triggered by the activation of the Ras-like small GTPase Sar1 by GDP/GTP exchange, and activated Sar1 in turn promotes COPII coat assembly. Subsequent GTP hydrolysis by Sar1 leads to disassembly of the coat proteins, which are then recycled for additional rounds of vesicle formation. Thus, the Sar1 GTPase cycle is thought to regulate COPII coat assembly and disassembly. Emerging evidence suggests that the cargo proteins modulate the Sar1 GTP hydrolysis to coordinate coat assembly with cargo selection. Here, I discuss the possible roles of the GTP hydrolysis by Sar1 in COPII coat assembly and selective uptake of cargo proteins into transport vesicles.