Association between the cell cycle and neural crest delamination through specific regulation of G1/S transition
Association between the cell cycle and neural crest delamination through specific regulation of G1/S transition
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DOI：
10.1016/s1534-5807(02)00221-6
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发表时间：
2002-09-01
影响因子：
11.8
通讯作者：
Kalcheim, C
中科院分区：
文献类型：
--
作者：
Burstyn-Cohen, T;Kalcheim, C
迁移前神经嵴细胞从背神经管的分层取决于环境信号和细胞内在机制，并且是细胞参与迁移的先决条件。在这里，我们发现鸟类神经嵴细胞在细胞周期的 S 期从神经管同步迁移。此外，在卵子和外植体中对从 G1 到 S 的转变的特异性抑制可以阻止分层，而在 S 或 G2 期的停滞则没有立即效果。因此，G1期间发生的控制从G1到S的转变的事件对于嵴前体的上皮向间质转化是必要的。
Delamination of premigratory neural crest cells from the dorsal neural tube depends both upon environmental signals and cell-intrinsic mechanisms and is a prerequisite for cells to engage in migration. Here we show that avian neural crest cells synchronously emigrate from the neural tube in the S phase of the cell cycle. Furthermore, specific inhibition of the transition from G1 to S both in ovo and in explants blocks delamination, whereas arrest at the S or G2 phases has no immediate effect. Thus, the events taking place during G1 that control the transition from G1 to S are necessary for the epithelial to mesenchymal conversion of crest precursors.