Connexin32 is expressed in vascular endothelial cells and participates in gap-junction intercellular communication
Connexin32 is expressed in vascular endothelial cells and participates in gap-junction intercellular communication
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DOI：
10.1016/j.bbrc.2009.02.148
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发表时间：
2009-05-01
影响因子：
3.1
通讯作者：
Suzuki, Koji
中科院分区：
文献类型：
--
作者：
Okamoto, Takayuki;Akiyama, Mari;Suzuki, Koji
内皮细胞（EC）在血管生物学中起着许多作用，包括控制血压、凝血、动脉粥样硬化、血管生成和炎症。间隙连接（GJ）是连接蛋白（Cx）家族蛋白的通道样组装体，其通过细胞内介质的转移连接相邻细胞并调节和同步它们的细胞内环境。已有报道血管内皮细胞表达Cx37、Cx40和Cx43，但不表达Cx32。在这里，我们表明，Cx32 mRNA和蛋白质表达在各种培养的人EC。我们使用野生型和Cx32基因敲除小鼠证实了Cx32在血管内皮细胞中的表达。我们观察到，通过间隙连接的培养的EC之间的染料转移被抑制的抗Cx32单克隆抗体。这些结果表明，血管内皮细胞表达Cx32，参与内皮细胞间隙连接细胞间通讯。(C)2009 Elsevier Inc. All rights reserved.
Endothelial cells (ECs) play many roles in vascular biology, including control of blood pressure, blood clotting, atherosclerosis, angiogenesis, and inflammation. Gap junctions (GJs) are channel-like assemblies of connexin (Cx) family proteins that connect neighboring cells and modulate and synchronize their intracellular environments by the transfer of intracellular mediators. It has been reported that vascular ECs express Cx37, Cx40, and Cx43, but not Cx32. Here, we showed that Cx32 mRNA and protein are expressed in various cultured human ECs. We confirmed Cx32 expression in blood vessel ECs using wild-type and Cx32 knock-out mice. We observed that dye transfer between cultured ECs through gap junctions is suppressed by an anti-Cx32 monoclonal antibody. These findings Suggest that vascular ECs express Cx32, which participates in endothelial gap-junction intercellular communication. (C) 2009 Elsevier Inc. All rights reserved.