Induction of donor-specific ACAID can prolong orthotopic corneal allograft survival in "high-risk" eyes.
Induction of donor-specific ACAID can prolong orthotopic corneal allograft survival in "high-risk" eyes.
复制标题
诱导供体特异性 ACAID 可以延长“高危”眼的同种异体原位角膜移植物的存活率。
DOI：
10.1076/ceyr.16.11.1171.5109
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发表时间：
1997
影响因子：
2
通讯作者：
Streilein,JW
中科院分区：
文献类型：
--
作者：
Sano,Y;Okamoto,S;Streilein,JW
方法：将C57 BL/6小鼠腹腔渗出液细胞（PEC）与转化生长因子（TGF）-β共同孵育过夜，观察供体特异性前房相关免疫偏离（ACAID）诱导对新生血管移植床中同种异体角膜移植物存活的影响。将培养的PEC静脉内（iv）注射到BALB/c小鼠中，1周后，这些动物接受来自C57 BL/6供体的原位角膜同种异体移植到新生血管化移植床上。对照小鼠接受同系（BALB/c）PEC的静脉内注射，与TGF-β 1一起培养过夜，然后接受来自C57 BL/6供体的原位角膜同种异体移植物。然而，只有6/16（37.5%）的角膜同种异体移植物被拒绝的受体，其中供体特异性ACAID已诱导注射同种异体PEC培养TGF-β.CONCLUSIONPrevious研究表明，排斥反应原位角膜移植物在新生血管移植床小鼠与收购供体特异性迟发性超敏反应（DH）。这项研究的结果表明，诱导供体特异性ACAID，选择性地削弱DH对供体抗原的反应，有效地降低了“高危”眼角膜移植物的存活率。
PURPOSETo examine the effect of donor-specific anterior chamber-associated immune deviation (ACAID) induction on the survival of orthotopic corneal allografts in neovascularized graft beds.METHODSTo induce donor-specific ACAID in recipients, peritoneal exudate cells (PEC) from C57BL/6 mice were incubated overnight with transforming growth factor (TGF)-ß. Cultured PEC were injected intravenously (iv) into BALB/c mice, and, 1 week later, these animals received orthotopic corneal allografts from C57BL/6 donors into neovascularized graft beds. Control mice received iv injection of syngeneic (BALB/c) PEC, cultured overnight with TGF-ß, and then received orthotopic corneal allografts from C57BL/6 donors.RESULTSAll corneal allografts (15 out of 15) were rejected within 2 weeks after grafting in the neovascularized graft beds of control animals. However, only 6 out of 16 (37.5%) of corneal allografts were rejected in recipients in which donor-specific ACAID had been induced by injection of allogeneic PEC cultured with TGF-ß.CONCLUSIONPrevious studies revealed that rejection of orthotopic corneal allografts in neovascularized graft beds in mice correlated with acquisition of donor-specific delayed hypersensitivity (DH). The results of this study suggest that induction of donor-specific ACAID, which selectively impairs DH responses to donor antigens, effectively prolongs corneal allograft survival in "high-risk" eyes.