LIM and SH3 protein 1, a promoter of cell proliferation and migration, is a novel independent prognostic indicator in hepatocellular carcinoma
LIM and SH3 protein 1, a promoter of cell proliferation and migration, is a novel independent prognostic indicator in hepatocellular carcinoma
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LIM和SH3蛋白1是细胞增殖和迁移的促进剂，是肝细胞癌的新型独立预后指标
DOI：
10.1016/j.ejca.2012.09.032
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发表时间：
2013-03-01
影响因子：
8.4
通讯作者：
Zhao, Liang
中科院分区：
文献类型：
--
作者：
Wang, Hui;Li, Weidong;Zhao, Liang
LIM和SH 3蛋白1（LASP-1）在几种恶性肿瘤的形成中起重要作用。然而，LASP-1在肝细胞癌（HCC）中的生物学和临床意义仍不清楚。采用免疫组化方法，我们分析了LASP-1在144例具有临床病理特征的HCC病例中的表达。利用基因转染和RNA干扰，我们研究了LASP-1过表达和耗竭对体外肿瘤细胞行为的影响。LASP-1在肝癌和肝细胞中不仅表达于胞浆，而且表达于胞核。LASP-1在肝癌组织胞浆和胞核中的阳性表达率均高于癌旁组织。统计学分析表明，LASP-1的异质性表达与肝癌患者的B型肝炎表面抗原（HBsAg）和血清甲胎蛋白（AFP）水平有关。HCC中胞浆LASP-1过表达与临床预后恶化之间存在显著趋势。多因素生存分析显示，胞浆LASP-1表达被认为是患者生存的独立预后因素。体外研究表明，LASP-1可促进细胞增殖和迁移，并导致癌细胞的侵袭性表型。LASP-1是HCC进展的有价值的标志物。高胞浆LASP-1表达与HCC患者的总生存率相关。(C)2012爱思唯尔有限公司保留所有权利。
LIM and SH3 protein 1 (LASP-1) plays a significant role in the formation of several malignant tumours. However, the biological and clinical significances of LASP-1 in hepatocellular carcinoma (HCC) remain largely unknown. Using immunohistochemistry, we analysed LASP-1 expression in 144 clinicopathologically characterised HCC cases. Using gene and transfection and RNA interference, we investigated the effects of LASP-1 overexpression and depletion on tumour cellular behaviour in vitro. LASP-1 expression was detected in not only cytoplasm and but also nucleus of HCC and liver cells. The positive rates of both cytosolic and nuclear LASP-1 expression in HCC were higher than adjacent non-tumourous tissues. Statistical analysis showed that heterogeneous LASP-1 expression is associated with hepatitis B surface antigen (HBsAg) and serum alpha-fetoprotein (AFP) level of HCC patients. A significant trend was identified between cytosolic LASP-1 overexpression in HCC and worsening clinical prognosis. Multivariate survival analysis showed that cytosolic LASP-1 expression was recognised as an independent prognostic factor of patient's survival. In vitro study showed LASP-1 promoted cell proliferation and migration, and resulted in aggressive phenotypes of cancer cells. LASP-1 is a valuable marker of HCC progression. High cytosolic LASP-1 expression is associated with poor overall survival in HCC patients. (C) 2012 Elsevier Ltd. All rights reserved.