Involvement of two type 2C protein phosphatases BcPtc1 and BcPtc3 in the regulation of multiple stress tolerance and virulence of Botrytis cinerea.
Involvement of two type 2C protein phosphatases BcPtc1 and BcPtc3 in the regulation of multiple stress tolerance and virulence of Botrytis cinerea.
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DOI：
10.1111/1462-2920.12126
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发表时间：
2013-10
影响因子：
5.1
通讯作者：
Qianqian Yang;Jinhua Jiang;C. Mayr;M. Hahn;Zhonghua Ma
中科院分区：
文献类型：
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作者：
Qianqian Yang;Jinhua Jiang;C. Mayr;M. Hahn;Zhonghua Ma
2C型丝氨酸/苏氨酸磷酸酶（PP 2Cs）在许多真核生物中参与多种细胞过程，但在丝状真菌中的功能尚不清楚。灰葡萄孢含有4个PP 2C基因，分别命名为BcPTC 1、BcPTC-3、BcPTC-5和BcPTC-6。通过基因缺失和互补分析这些基因的生物学功能。BcPTC 5和BcPTC 6的突变体没有观察到与野生型不同的表型，而BcPTC 1和BcPTC 3的突变体具有减少的菌丝生长、增加的分生孢子和受损的菌核发育。此外，BcPTC 1和BcPTC 3突变体表现出增加的敏感性，渗透和氧化应激，细胞壁降解酶。这两个突变体表现出显着降低对寄主植物组织的毒力。所有的缺陷都通过突变体分别与野生型BcPTC 1和BcPTC 3的遗传互补而恢复。与酿酒酵母中已知的不同，BcPtc 3，而不是BcPtc 1，负调控BcSak 1（S.酿酒酵母Hog 1）。灰霉病，虽然BcPTC 1和BcPTC 3都能够挽救酵母PTC 1缺失突变体在各种应激条件下的生长缺陷。这些结果表明，BcPtc 1和BcPtc 3在调节B的多重胁迫耐受性和毒力方面起重要作用。灰绿色
Type 2C Ser/Thr phosphatases (PP2Cs) are involved in various cellular processes in many eukaryotes, but little has been known about their functions in filamentous fungi. Botrytis cinerea contains four putative PP2C genes, named BcPTC1, -3, -5, and -6. Biological functions of these genes were analysed by gene deletion and complementation. While no phenotypes aberrant from the wild type were observed with mutants of BcPTC5 and BcPTC6, mutants of BcPTC1 and BcPTC3 had reduced hyphal growth, increased conidiation, and impaired sclerotium development. Additionally, BcPTC1 and BcPTC3 mutants exhibited increased sensitivity to osmotic and oxidative stresses, and to cell wall degrading enzymes. Both mutants exhibited dramatically decreased virulence on host plant tissues. All of the defects were restored by genetic complementation of the mutants with wild-type BcPTC1 and BcPTC3 respectively. Different from what is known in Saccharomyces cerevisiae, BcPtc3, but not BcPtc1, negatively regulates phosphorylation of BcSak1 (the homologue of S. cerevisiae Hog1) in B. cinerea, although both BcPTC1 and BcPTC3 were able to rescue the growth defects of a yeast PTC1 deletion mutant under various stress conditions. These results demonstrated that BcPtc1 and BcPtc3 play important roles in the regulation of multiple stress tolerance and virulence of B. cinerea.