Changes in the photosynthetic characteristics and photosystem stoichiometries in wild-type and Chl b-deficient mutant rice seedlings under various irradiances
Changes in the photosynthetic characteristics and photosystem stoichiometries in wild-type and Chl b-deficient mutant rice seedlings under various irradiances
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DOI：
10.1007/s11099-010-0069-5
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发表时间：
2010-12
期刊：
影响因子：
2.7
通讯作者：
J. Yamazaki
中科院分区：
文献类型：
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作者：
J. Yamazaki
以野生型水稻（Oryza sativa L.）CV.本研究以叶绿素（Chl）b缺失突变体（Chlorina 11）和叶绿素8号突变体（Chlorina 8）为材料，测定了叶绿素b缺失突变体（Chlorina 11）叶片的放氧率、叶绿素b、β-胡萝卜素和叶黄素含量、细胞色素f含量以及类囊体中光系统I（PSI）和光系统II（PSII）的反应中心。在5%和2%的全光照条件下，诺林8号和小绿11的光合放氧率、PS Ⅱ最大量子效率（Fv/Fm）和叶绿素浓度在6 d内基本保持不变。另一方面，在类囊体水平上，叶绿素11的PSII反应中心更稳定，即使在高光照下，而约40%的水平下降的PSII反应中心发生在2%的全光照下6天。然而，在这样的条件下，通过调节活性PSII和PSI中心的化学计量比，在两个水稻类型的光吸收平衡之间的两个光系统进行调整。本研究试图研究是否PSII和PSI之间的光吸收平衡被改变，以有效地进行光合作用在野生型和叶绿素b缺失突变体水稻幼苗。
By using a wild-type rice (Oryza sativaL. cv. Norin No. 8) and the chlorophyll (Chl)b-deficient mutant derived from Norin No. 8 (chlorina 11), the present study monitored the oxygen evolution, contents of Chlaandb, β-carotene, and lutein in leaf and the contents of cytochromef, and the reaction centres of photosystem I (PSI) and photosystem II (PSII) in thylakoids. The oxygen evolution, maximal quantum yield of PSII (Fv/Fm) and Chl concentration remained constant in both Norin No. 8 and chlorina 11 under 5 and 2% of full sunlight for six days. On the other hand, on the thylakoid level, the PSII reaction centre of chlorina 11 was more stable even under high irradiance, while approximately 40% decrease in levels of the PSII reaction centre occurred under 2% of full sunlight for six days. However, under such conditions, by regulating the stoichiometry of active PSII and PSI centres, the light absorption balance in both rice types was adjusted between the two photosystems. The present study attempted to examine whether the light absorption balance between PSII and PSI is altered to effectively conduct photosynthesis in the wild-type and Chlb-deficient mutant rice seedlings.