The ylbO gene product of Bacillus subtilis is involved in the coat development and lysozyme resistance of spore
The ylbO gene product of Bacillus subtilis is involved in the coat development and lysozyme resistance of spore
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DOI：
10.1016/j.femsle.2004.10.038
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发表时间：
2005-01-01
影响因子：
2.1
通讯作者：
Watabe, K
中科院分区：
文献类型：
--
作者：
Kuwana, R;Okumura, T;Watabe, K
枯草芽孢杆菌YlbO蛋白是在SigE控制下表达的含有Myb样DNA结合结构域的蛋白。在这里，我们分析了ylbO阴性细胞中的基因表达和蛋白质组成。SDS-PAGE分析显示ylbO阴性孢子的蛋白质谱不同于野生型。具体地，外壳蛋白CgeA、CotG和CotY的表达。由SigK和GerE控制的蛋白质在ylbO阴性细胞中减少。北方印迹分析表明YlbO调控cgeA、cotG和cotY的转录。这些结果表明，在B孢子形成过程中，YlbO调节一些外壳蛋白的表达。枯草杆菌直接或间接。(C)2004年，欧洲微生物学会联合会。Elsevier B. V.出版，保留所有权利。
The Bacillus subtilis YlbO protein is a Myb-like DNA binding domain-containing protein that is expressed under the control of SigE. Here, we analyzed gene expression and protein composition in ylbO-negative cells. SDS-PAGE analysis revealed that the protein profile of ylbO- negative spores differed from that of wild-type. Specifically, the expression of coat proteins CgeA, CotG, and CotY. which are controlled by SigK and GerE, was reduced in ylbO -negative cells. Northern blot analysis revealed that YlbO regulated the transcription of cgeA, cotG, and cotY. These results suggest that YlbO regulates the expression of some coat proteins during sporulation in B. subtilis directly or indirectly. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.