Constitutive Expression of RbAp 46 Induces Epithelial-mesenchymal Transition in Mammary Epithelial Cells
Constitutive Expression of RbAp 46 Induces Epithelial-mesenchymal Transition in Mammary Epithelial Cells
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RbAp 46 的组成型表达诱导乳腺上皮细胞上皮-间质转化
DOI：
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发表时间：
2008
期刊：
影响因子：
--
通讯作者：
Zhaofeng Wang
中科院分区：
文献类型：
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作者：
Liang Guan;Zhaofeng Wang
背景：上皮-间质转化（EMT）是胚胎发育过程中的一个重要组成部分。EMT是一个受到严格调控的过程，其异常调控可能有助于癌症的进展和转移。视网膜母细胞瘤抑制因子相关蛋白46（Rb-associated protein 46，RbAp 46）是组蛋白修饰和重塑复合物的一个组成部分。材料与方法：免疫印迹和免疫荧光法与上皮和间充质标记和细胞迁移和侵袭测定。结果如下：表达重组RbAp 46的乳腺上皮细胞MCF 10AT 3B表现出与上皮标志物下调和间充质标志物上调相关的EMT，以及增加的迁移和侵袭表型。结论：Rbap 46可能是胚胎发育过程中EMT的重要调控因子。RbAp 46表达失调可能导致癌症进展，
Background: Epithelial-mesenchymal transition (EMT) is a component of several morphogenetic and organogenetic processes during embryonic development. EMT is a strictly regulated process and its aberrant regulation may contribute to cancer progression and metastasis. The retinoblastoma suppressor (Rb)-associated protein 46 (RbAp46) is a component of the histone-modifying and -remodeling complexes. Materials and Methods: Western blot and immunofluorescent assays with epithelial and mesenchymal markers and cell migration and invasion assays were employed. Results: Mammary epithelial cells, MCF10AT3B, that express recombinant RbAp46 exhibited an EMT associated with the down-regulation of epithelial markers and up-regulation of mesenchymal markers, as well as an increased migration and invasion phenotype. Conclusion: Rbap46 may be an important player in EMT during embryonic development. Dysregulated RbAp46 expression may contribute to cancer progression and
DOI：
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发表时间：
1996
期刊：
The American journal of pathology
影响因子：
--
作者：
P. Dawson;S. Wolman;L. Tait;G. Heppner;F. Miller
通讯作者：
P. Dawson;S. Wolman;L. Tait;G. Heppner;F. Miller
影响因子：
10.5
作者：
Zhang, Y;Ng, HH;Reinberg, D
通讯作者：
Reinberg, D