Fluorescence analysis of 6-mercaptopurine with the use of a nano-composite consisting of BSA-capped Au nano-clusters and core-shell Fe3O4-SiO2 nanoparticles.
Fluorescence analysis of 6-mercaptopurine with the use of a nano-composite consisting of BSA-capped Au nano-clusters and core-shell Fe3O4-SiO2 nanoparticles.
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DOI：
10.1016/j.bios.2015.03.035
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发表时间：
2015-08
影响因子：
12.6
通讯作者：
Zhuo Li;Yong Wang;Y. Ni;S. Kokot
中科院分区：
文献类型：
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作者：
Zhuo Li;Yong Wang;Y. Ni;S. Kokot
A magnetic and fluorescent nano-composite was prepared. It comprised of a core of Fe3O4nanoparticles (NPs), a silica shell and satellitic Au nano-clusters (AuNCs) capped with bovine serum albumin (BSA). This nano-composite has many desirable properties, e.g. magnetism, red emission, high water solubility, and high resistance to photo-bleaching. On addition of the analyte, 6-mercaptopurine (6-MP) or indeed other similar thiols, AuNCs formed aggregates because the existing cross-links within the Fe3O4NPs@SiO2and AuNC structure were broken in favor of the gold–thiol bonds. On suitable irradiation of such aggregates, red fluorescence was emitted at 613 nm. It decreased significantly as a function of the added 6-MP concentration, and the quenching ratio (F0–F) /F0was related linearly to the concentration of 6-MP in the range of 0.01 to 0.5 μmol L−1. The detection limit was 0.004 μmol L−1(S/N=3). The method was strongly selective for 6-MP in the presence of oxidants, phenols, heavy-metal ions, and especially bio-thiols.
