Gene expression in juvenile arthritis and spondyloarthropathy:: pro-angiogenic ELR+ chemokine genes relate to course of arthritis
Gene expression in juvenile arthritis and spondyloarthropathy:: pro-angiogenic ELR+ chemokine genes relate to course of arthritis
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DOI：
10.1093/rheumatology/keh224
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发表时间：
2004-08-01
期刊：
影响因子：
5.5
通讯作者：
Thompson, SD
中科院分区：
文献类型：
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作者：
Barnes, MG;Aronow, BJ;Thompson, SD
Objective.评估基于微阵列的方法鉴定幼年型关节炎患者和健康对照者外周血单个核细胞（PBMC）和滑液单个核细胞（SFMC）中具有疾病特异性表达模式的基因的能力。分析了从患有活动性疾病的患者（根据ACR标准按病程分类）收集的26个PBMC和20个SFMC样品的微阵列数据（Affyellow U95Av2）与疾病特征相关的表达模式。为了比较，从15名健康对照获得PBMC基因表达谱。实时荧光定量PCR用于确认基因表达差异。对PBMC中基因表达模式的统计分析确定了378个探针集，对应于多关节病程患者和对照组之间表达水平不同的342个独特基因（t检验，P
Objective. To evaluate the ability of microarray-based methods to identify genes with disease-specific expression patterns in peripheral blood mononuclear cells (PBMC) and synovial fluid mononuclear cells (SFMC) of juvenile arthritis patients and healthy controls.Methods. Microarray data (Affymetrix U95Av2) from 26 PBMC and 20 SFMC samples collected from patients with active disease (classified by course according to ACR criteria) were analysed for expression patterns that correlated with disease characteristics. For comparison, PBMC gene expression profiles were obtained from 15 healthy controls. Real-time PCR was used for confirmation of gene expression differences.Results. Statistical analysis of gene expression patterns in PBMC identified 378 probe sets corresponding to 342 unique genes with differing expression levels between polyarticular course patients and controls (t test, P