Eosinophilic rhinitis accompanies the development of lower airway inflammation and hyper-reactivity in sensitized mice exposed to aerosolized allergen
Eosinophilic rhinitis accompanies the development of lower airway inflammation and hyper-reactivity in sensitized mice exposed to aerosolized allergen
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DOI：
10.1046/j.1365-2222.2001.01081.x
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发表时间：
2001-05-01
影响因子：
6.1
通讯作者：
Ceuppens, JL
中科院分区：
文献类型：
--
作者：
Hellings, PW;Hessel, EM;Ceuppens, JL
背景 过敏性鼻炎是哮喘发展的危险因素。大约80%的哮喘患者同时患有鼻炎。然而，过敏性鼻炎和哮喘的诱发模式仍不清楚。 目的 本研究的目的是调查小鼠在哮喘样疾病发生过程中以及急性过敏原激发后上呼吸道炎症的发展情况。 方法 BALB-c 小鼠腹膜内 (i.p) 对卵清蛋白 (OA，第 1-13 天) 致敏，并连续 8 天 (days) 用 OA 或盐水气溶胶激发。 33-40）。在第二个实验中，慢性暴露 8 天，然后休息 10 天，然后进行急性雾化过敏原激发（第 50 天）。观察不同时间点的炎症参数。结果通过鼻腔和支气管肺泡灌洗液以及鼻和支气管的组织学切片分析显示，在重复吸入雾化OA的过程中，同时诱发了上、下气道炎症。磁共振图像（MRI）扫描显示，出现支气管高反应性的小鼠鼻粘膜厚度也增加。当慢性暴露后发生急性过敏原激发时，后者会导致全身性 IL-5 水平升高，同时血液和气道嗜酸性粒细胞（主要是鼻子）升高。 结论 在呼吸道过敏小鼠模型中发现，鼻子和肺部同时诱导嗜酸性粒细胞炎症。这些发现支持这样的观点，即上气道和下气道疾病代表涉及一个共同气道的一系列炎症，并为吸入过敏原后全身气道炎症的概念提供了证据。
Background Allergic rhinitis is a risk factor for the development of asthma. About 80% of asthmatic patients also have rhinitis. However, the pattern of induction of allergic rhinitis and asthma remains unclear.Objective The purpose of this study was to investigate the development of upper airway inflammation in mice during the development of an asthma-like disease and after an acute allergen provocation.Methods BALB-c mice were sensitized intraperitoneally (i.p) to ovalbumin (OA, days 1-13) and were challenged with aerosols of either OA or saline on 8 consecutive days (days 33-40). In a second experiment, chronic exposure for 8 days was followed by 10 days of rest and then an acute nebulized allergen provocation was performed (day 50). Inflammatory parameters were investigated at different time-points.Results Upper and lower eosinophilic airway inflammation were simultaneously induced in the course of repeated inhalations of nebulized OA, as shown by analyses of nasal and broncho-alveolar lavage fluids and histological sections of the nose and bronchi. Mice that developed bronchial hyper-responsiveness also had increased thickness of the nasal mucosa on magnetic resonance image (MRI) scans. When chronic exposure was followed by acute allergen provocation, the latter caused a systemic increase in IL-5 levels, with a concomitant rise in blood and airway eosinophils, primarily in the nose.Conclusions Simultaneous induction of eosinophilic inflammation in the nose and lungs was found in a mouse model of respiratory allergy. These findings support the viewpoint that upper and lower airway disease represent a continuum of inflammation involving one common airway and provide evidence for the concept of global airway inflammation after inhalation of allergen.