Localized quorum sensing in Vibrio fischeri
Localized quorum sensing in Vibrio fischeri
复制标题
DOI：
10.1016/j.colsurfb.2007.09.031
复制
发表时间：
2008-04-01
影响因子：
5.8
通讯作者：
Velegol, Darrell
中科院分区：
文献类型：
--
作者：
Parent, Mary E.;Snyder, Charles E.;Velegol, Darrell
在细菌的三维大样本中，群体感应几乎总是被认为是一种种群密度效应。在这里，我们创建了粘在玻璃表面上的费氏弧菌细胞的二维样本，以检查当地人口密度对群体感应的影响。这是通过测量发光响应来实现的。2-D细菌种群使我们能够同时考虑时间和距离对群体感应的影响，以前在典型的三维大样本中获取这些影响是非常困难的。因此，我们能够从信号扩散的角度来考虑群体感知。群体感应信号的扩散模型指导实验，并表明对于给定的细胞间距(密度)和扩散时间，存在一个“真正的群体”--群体感知所需的细胞数量。我们发现群体感应可以在2-D表面样本中局部发生，并且是细胞密度和信号扩散时间的函数。(C)2007 Elsevier B.V.保留所有权利。
Quorum sensing is almost always regarded as a population density effect in three-dimensional bulk samples of bacteria. Here we create two-dimensional samples of Vibrio fischeri cells adhered onto glass surfaces to examine the effect of local population densities on quorum sensing. This is done by measuring the luminescent response. The 2-D bacterial populations enable us to simultaneously account for time and distance effects on quorum sensing, which were previously very challenging to access in typical three-dimensional bulk samples. Thus, we are able to consider quorum sensing in terms of signal diffusion. A diffusion model of quorum sensing signals guides the experiments and shows that for a given cell spacing (density) and diffusion time there exists a "true quorum"- a number of cells necessary for quorum sensing. We find that quorum sensing can occur locally in 2-D surface samples and is a function of cell population density as well as signal diffusion time. (C) 2007 Elsevier B.V. All rights reserved.