The Antimicrobial Peptide Gad‐1 Clears Pseudomonas aeruginosa Biofilms under Cystic Fibrosis Conditions
The Antimicrobial Peptide Gad‐1 Clears Pseudomonas aeruginosa Biofilms under Cystic Fibrosis Conditions
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DOI：
10.1002/cbic.202000816
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发表时间：
2021-01
期刊：
影响因子：
3.2
通讯作者：
Jasmin Portelinha;A. Angeles‐Boza
中科院分区：
文献类型：
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作者：
Jasmin Portelinha;A. Angeles‐Boza
囊性纤维化（CF）患者的细菌感染是一个新出现的健康问题，并导致过早死亡。CF是一种遗传性疾病，在肺部产生厚粘液，易于形成细菌生物膜，特别是铜绿假单胞菌生物膜。这些生物膜非常难以治疗，因为它们中的许多具有抗生素抗性，并且由于细胞外DNA（eDNA）的存在而恶化。eDNA有助于稳定生物膜，并可以结合抗菌化合物以减轻其影响。金属抗菌肽Gaduscidin-1（Gad-1）通过多种作用模式的组合根除已建立的铜绿假单胞菌生物膜，包括可以切割生物膜中eDNA的核酸酶活性。此外，当与抗生素卡那霉素和环丙沙星一起使用时，Gad-1表现出协同活性，从而使Gad-1成为一种新的先导化合物，可用于治疗CF患者的细菌生物膜。
Bacterial infections in cystic fibrosis (CF) patients are an emerging health issue and lead to a premature death. CF is a hereditary disease that creates a thick mucus in the lungs that is prone to bacterial biofilm formation, specifically Pseudomonas aeruginosa biofilms. These biofilms are very difficult to treat because many of them have antibiotic resistance that is worsened by the presence of extracellular DNA (eDNA). eDNA helps to stabilize biofilms and can bind antimicrobial compounds to lessen their effects. The metallo‐antimicrobial peptide Gaduscidin‐1 (Gad‐1) eradicates established P. aeruginosa biofilms through a combination of modes of action that includes nuclease activity that can cleave eDNA in biofilms. In addition, Gad‐1 exhibits synergistic activity when used with the antibiotics kanamycin and ciprofloxacin, thus making Gad‐1 a new lead compound for the potential treatment of bacterial biofilms in CF patients.