Intravitreous high expression level of netrin-1 in patients with proliferative diabetic retinopathy.
Intravitreous high expression level of netrin-1 in patients with proliferative diabetic retinopathy.
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DOI：
10.3969/j.issn.1000-4432.2011.02.017
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发表时间：
2011-06
期刊：
影响因子：
--
通讯作者：
Xu H
中科院分区：
文献类型：
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作者：
Liu J;Xia X;Xiong S;Le Y;Xu H
为探讨netrin-1和血管内皮生长因子（VEGF）在视网膜血管生成中的意义，检测了增殖性糖尿病视网膜病变（PDR）和非增殖性糖尿病视网膜病变（non-PDR）患者玻璃体液和血清中netrin-1和VEGF的水平。然后我们测定了netrin-1和VEGF在氧诱导视网膜病变（OIR）小鼠视网膜中的表达。我们的研究共包括18名患者的18只眼睛，其中10只来自PDR患者，8只来自非PDR患者。在睫状体平坦部玻璃体切除术期间收集未稀释的玻璃体液样品。如果可能，采集适当的血液样本。通过酶联免疫吸附试验测定玻璃体液和血浆中Netrin-1和VEGF的水平。建立OIR小鼠模型，免疫组化法检测netrin-1和VEGF的表达。PDR患者玻璃体中netrin-1和VEGF水平均显著高于对照组（Mediannetrin-1=509.94 vs.85.91pg/ml，P<0.001; MedianVEGF=762.60 vs.77.52pg/ml，P<0.001）。Netrin-1主要表达于小鼠视网膜的GCL和INL。netrin-1和VEGF在OIR小鼠中均上调。在PDR患者和OIR小鼠视网膜的玻璃体液中Netrin-1和VEGF水平升高。因此，netrin-1可能在病理性视网膜血管生成中发挥重要作用。
To investigate the significance of netrin-1 and vascular endothelial growth factor (VEGF) in the pathogenesis of retinal angiogenesis, the levels of netrin-1 and VEGF in the vitreous fluid and serum of the proliferative diabetic retinopathy (PDR) and non-proliferative diabetic retinopathy (non-PDR) patients were measured. We then determined the netrin-1 and VEGF expression in the oxygen induced retinopathy (OIR) mice retina. A total of 18 eyes from 18 patients were included in our study and 10 of them were collected from PDR patients and 8 from non-PDR patients. Undiluted vitreous fluid samples were collected during pars plana vitrectomy. Appropriate blood samples were collected if possible. Netrin-1 and VEGF levels in the vitreous fluid and plasma were determined by Enzyme-linked Immunosorbent Assays. OIR mice models were established, and netrin-1 and VEGF levels were determined by immunohistochemistry analysis. The levels of netrin-1 and VEGF in the vitreous of PDR patients were significantly higher than those in the controls (Mediannetrin-1=509.94 vs. 85.91 pg/ml, P<0.001 and MedianVEGF=762.60 vs. 77.52 pg/ml, P<0.001). Netrin-1 was mainly expressed in GCL and INL of the retina in mice. Both netrin-1 and VEGF were up-regulated in OIR mice. Netrin-1 and VEGF levels were elevated in vitreous fluid of the PDR patients and the OIR mice retina. Therefore, netrin-1 may play an important role in pathological retinal angiogenesis.