Phosphatidic acid mediates salt stress response by regulation of MPK6 in Arabidopsis thaliana
Phosphatidic acid mediates salt stress response by regulation of MPK6 in Arabidopsis thaliana
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DOI：
10.1111/j.1469-8137.2010.03422.x
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发表时间：
2010-01-01
期刊：
影响因子：
9.4
通讯作者：
Zhang, Wenhua
中科院分区：
文献类型：
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作者：
Yu, Lijuan;Nie, Jianing;Zhang, Wenhua
P>磷脂酶 D (PLD) 水解磷脂产生磷脂酸 (PA) 和头基，并参与对包括盐度在内的各种环境胁迫的响应。在这里，我们通过调节拟南芥中丝裂原激活蛋白激酶（MAPK或MPK）确定了PLDα和PA在介导盐（NaCl）应激信号传导中的作用。通过电喷雾电离串联质谱（ESI-MS/MS）定量分析了NaCl诱导的PA含量和组成的变化。根据过滤器结合和 ELISA，特定的 PA 种类（MAPK 16:0-18:2 PA）通过暴露于 NaCl 而丰度增加，与 MPK6 结合。使用凝胶内分析测试 PA 对 MPK6 活性的影响。16:0-18:2 PA 刺激拟南芥叶提取物免疫沉淀的 MPK6 活性。氯化钠处理诱导野生型植物中 MPK6 的瞬时激活，但在 pld α 1 植物突变体中该激活被消除。质膜 Na+/H+ 逆向转运蛋白 (SOS1) 被确定为 MPK6 的下游靶标。 MPK6 磷酸化 SOS1 的 C 端片段。 SOS1 的 MPK6 磷酸化受到 NaCl 处理以及直接 PA 的刺激。这些结果表明，PA 在响应盐胁迫的耦合 MAPK 级联中发挥着关键作用。
P>Phospholipase D (PLD) hydrolyzes phospholipids to produce phosphatidic acid (PA) and a head group, and is involved in the response to various environmental stresses, including salinity. Here, we determined the roles of PLD alpha and PA in the mediation of salt (NaCl)-stress signaling through the regulation of mitogen-activated protein kinase (MAPK or MPK) in Arabidopsis thaliana.NaCl-induced changes in the content and composition of PA were quantitatively profiled by electrospray ionization-tandem mass spectrometry (ESI-MS/MS). A specific PA species (a MAPK 16:0-18:2 PA), which was increased in abundance by exposure to NaCl, bound to a MPK6, according to filter binding and ELISA. The effect of PA on MPK6 activity was tested using in-gel analysis.16:0-18:2 PA stimulated the activity of MPK6 immunoprecipitated from Arabidopsis leaf extracts. Treatment with NaCl induced a transient activation of MPK6 in wild-type plant, but the activation was abolished in the pld alpha 1 plant mutant. A plasma membrane Na+/H+ antiporter (SOS1) was identified as a downstream target of MPK6. MPK6 phosphorylated the C-terminal fragment of SOS1. The MPK6 phosphorylation of SOS1 was stimulated by treatment with NaCl, as well as directly by PA.These results suggest that PA plays a critical role in coupling MAPK cascades in response to salt stress.