Multiparameter Phenotyping of Human PBMCs Using Mass Cytometry.
Multiparameter Phenotyping of Human PBMCs Using Mass Cytometry.
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DOI：
10.1007/978-1-4939-2963-4_7
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发表时间：
2015
期刊：
影响因子：
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通讯作者：
Maecker HT
中科院分区：
文献类型：
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作者：
Leipold MD;Newell EW;Maecker HT
近几十年来，单细胞表型和功能分析的标准是荧光流式细胞术。质谱细胞仪是一种较新的技术，它使用重金属离子，而不是荧光染料，作为探针（如抗体）的标记。这些离子标记的探针与细胞的结合通过质谱法定量。这大大增加了可以同时探测的表型和功能标记的数量。在这里，我们回顾了在将现有流式细胞仪面板调整为质谱分析时必须考虑的主题。我们提出了一个协议和代表性的面板表面表型和细胞内细胞因子染色（ICS）测定。
The standard for single-cell analysis of phenotype and function in recent decades has been fluorescence flow cytometry. Mass cytometry is a newer technology that uses heavy metal ions, rather than fluorochromes, as labels for probes such as antibodies. The binding of these ion-labeled probes to cells is quantitated by mass spectrometry. This greatly increases the number of phenotypic and functional markers that can be probed simultaneously. Here, we review topics that must be considered when adapting existing flow cytometry panels to mass cytometry analysis. We present a protocol and representative panels for surface phenotyping and intracellular cytokine staining (ICS) assays.