Comparative Proteomics of Peritrophic Matrix Provides an Insight into its Role in Cry1Ac Resistance of Cotton Bollworm Helicoverpa armigera
Comparative Proteomics of Peritrophic Matrix Provides an Insight into its Role in Cry1Ac Resistance of Cotton Bollworm Helicoverpa armigera
复制标题
围食基质的比较蛋白质组学深入了解其在棉铃虫 Helicoverpa armigera 的 Cry1Ac 抗性中的作用
DOI：
10.3390/toxins11020092
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发表时间：
2019-02-01
期刊：
影响因子：
4.2
通讯作者：
Xiao, Yutao
中科院分区：
文献类型：
--
作者：
Jin, Minghui;Liao, Chongyu;Xiao, Yutao
来自苏云金芽孢杆菌（Bt）的晶体（Cry）蛋白被广泛用于喷雾剂和转基因作物以控制害虫，但昆虫抗性的进化威胁着它们的长期使用。不同的耐药机制已被确定，但有些尚未完全阐明。本文通过对棉铃虫XJ10中肠转录组和围食基质蛋白质组的比较分析，探讨棉铃虫对Cry1Ac的抗性机制。该菌株对Cry1Ac原毒素的抗性是XJ株的146倍，对Cry1Ac激活毒素的抗性是XJ株的45倍。与敏感菌株XJ相比，XJ10中几个胰蛋白酶基因的mRNA和蛋白水平下调。此外，还鉴定了PM的215种蛋白质，几乎所有蛋白质在中肠中都有相应的mRNA。这些结果提供了新的见解，PM可能参与Bt抗性。
Crystalline (Cry) proteins from Bacillus thuringiensis (Bt) are widely used in sprays and transgenic crops to control insect pests, but the evolution of insect resistance threatens their long-term use. Different resistance mechanisms have been identified, but some have not been completely elucidated. Here, the transcriptome of the midgut and proteome of the peritrophic matrix (PM) were comparatively analyzed to identify potential mechanism of resistance to Cry1Ac in laboratory-selected strain XJ10 of Helicoverpa armigera. This strain had a 146-fold resistance to Cry1Ac protoxin and 45-fold resistance to Cry1Ac activated toxin compared with XJ strain. The mRNA and protein levels for several trypsin genes were downregulated in XJ10 compared to the susceptible strain XJ. Furthermore, 215 proteins of the PM were identified, and nearly all had corresponding mRNAs in the midgut. These results provide new insights that the PM may participate in Bt resistance.