Interaction between GIC and S-sanguis biofilms:: Antibacterial properties and changes of surface hardness
Interaction between GIC and S-sanguis biofilms:: Antibacterial properties and changes of surface hardness
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DOI：
10.1016/j.jdent.2005.02.011
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发表时间：
2006-09-01
影响因子：
4.4
通讯作者：
Wilson, M.
中科院分区：
文献类型：
--
作者：
Hengtrakool, C.;Pearson, G. J.;Wilson, M.
目的：本研究的目的是调查血链球菌与两种玻璃离子制剂（GIC：A含氟和GIC：B不含氟）的相互作用，特别是参考细菌的生长和骨水泥的硬度随时间的变化。材料和方法：根据制造商的说明制备两种水活化玻璃离子水泥的光盘。使用羟基磷灰石盘（HA）作为对照。三维激光扫描技术用于表征生长生物膜之前的基底的表面粗糙度和面积。在生物膜生长之前和之后评估表面硬度。采用恒深膜发酵罐系统（CDFF）培养S。以类似于Wilson等人在1995年描述的方式在标本上形成血生物膜。对于敏感性测量，在第一次细菌定植后长达14天的时间内，将样本从CDFF中无菌取出。测量每个表面上积累的生物膜层中的活细菌计数，并将其转化为每单位表面积的菌落形成单位。为了确定储存介质的效果，将硬度盘暴露于蒸馏水、pH 4的乳酸、pH 5的乳酸、pH 5的柠檬酸、人工唾液和S.血生物膜制备和随后高压灭菌后24小时，将样本转移至含有40 ml储存培养基的容器中。结果：在7 d内，S. GIC：A组在前5d内每mm（2）的细胞数显著低于HA组和GIC：B组（p < 0.05）。与HA相比，GIC：B表面上的活菌计数在最初的5 d期间较低。GIC：A和GIC：B在不同介质中的暴露对水泥表面产生软化。很明显，生物膜的效果显著大于在水中的储存，但与在pH 5的乳酸中的储存相似。结论：本研究表明，S。两种玻璃离聚物制剂均显著影响血生物膜，在含氟化物的GIC表面上观察到更大的减少。S. Santiago生物膜使粘固剂的表面硬度降低，与在pH 5的乳酸中浸泡后所见的相同。这表明，S.血生物膜受到GIC的影响，水泥硬度也降低，表明水泥发生了一定程度的降解。(c)2006爱思唯尔有限公司保留所有权利。
Objective: The aim of this study was to investigate the interaction of Streptococcus sanguis with two glass-ionomer formulations (GIC:A containing fluoride and GIC:B without fluoride) with particular reference to bacterial growth and changes in hardness of the cement with respect to time.Materials and methods: Discs of two water activated glass-ionomer cements were prepared according to the manufacturer's instruction. Hydroxyapatite discs (HA) were used as controls. 3D laser scanning technique was used to characterize surface roughness and area of the substrate prior to growing biofilms. Surface hardness was evaluated before and after biofilm growth. A constant depth film fermenter system (CDFF) was used to grow S. sanguis biofilms on the specimens in a similar manner to that described previously by Wilson et al. in 1995. For susceptibility measurement, specimens were removed from CDFF aseptically over periods up to 14 d after the first colonization with bacteria. Counts of viable bacterial in the accumulating biofilm layer on each surface were measured and converted to colony forming units per unit surface area. To determine the effect of storage media, hardness discs were exposed to distilled water, lactic acid pH 4, lactic acid pH 5, citric acid pH 5, artificial saliva and S. sanguis biofilms. Twenty-four hours after preparing and subsequent autoclaving, specimens were transferred to a vessel containing 40 ml storage medium. The specimens were investigated for periods up to 7 d.Results: The viable counts of S. sanquis per mm(2) on GIC:A were significantly less than those on HA and GIC:B during the first 5 d (p < 0.05). The viable counts of bacteria on the surface of GIC:B were lower during the initial 5 d when compared to HA. Exposure of GIC:A and GIC:B to different medium produced softening to the surface of cement. it is apparent that the effects of the biofilms are significantly greater than storage in water but similar to storage in lactic acid pH 5. Conclusions: This investigation showed that the growth of S. sanguis biofilms were significantly affected by both glass-ionomer formulations, the greater reduction being noted on the surface of the fluoride containing GIC. S. sanquis biofilms produced reduction on the surface hardness of the cement equivalent to that seen after immersion in lactic acid at pH 5. This indicates that while S. sanguis biofilm is affected by the GIC, there is also a decrease in hardness of the cement indicating some cement degradation. (c) 2006 Elsevier Ltd. All rights reserved.