Properties and antityrosinase activity of sericin from various extraction methods
Properties and antityrosinase activity of sericin from various extraction methods
复制标题
DOI：
10.1042/ba20090186
复制
发表时间：
2010-02-01
影响因子：
2.8
通讯作者：
Srichana, Teerapol
中科院分区：
文献类型：
--
作者：
Aramwit, Pornanong;Damrongsakkul, Siriporn;Srichana, Teerapol
研究了不同提取方法从不同品种的泰国蚕丝中提取的丝素的化学性质和抗酪氨酸酶活性。不同的蚕丝品系含有不同的SS，具有不同的氨基酸组成，其显著地受所使用的提取方法的影响。尿素提取SS是唯一的方法，提供了清晰可辨的带，并有最显着的影响SS构象所示的FTIR（傅里叶变换红外）光谱。在提取过程中使用尿素或酸性或碱性化学品也会影响SS的热行为。在生物活性方面，用尿素提取的SS表现出最高的抗酪氨酸酶活性，而碱降解的SS没有表现出蘑菇酪氨酸酶的抑制作用。色素，主要是黄酮类化合物和类胡萝卜素从蚕茧，也被发现增强酪氨酸酶抑制SS。
The present study investigated the chemical properties and antityrosinase activities of SS (silk sericin) extracted from different Thai silk strains via various extraction methods. Different silk strains contain distinct SS with various amino acid compositions, which are significantly influenced by the extraction method used. Urea extraction of SS was the only method that provided clearly distinguishable bands and had the most significant impact on SS conformation as illustrated by FTIR (Fourier-transform infrared) spectra. The use of urea or either acidic or alkaline chemicals in the extraction process also influenced SS thermal behaviour. With regard to biological activity, SS extracted using urea exhibited the highest antityrosinase activity, whereas alkali-degraded SS showed no inhibition of mushroom tyrosinase. Pigments, primarily flavonoids and carotenoids from silk cocoons, were also found to enhance tyrosinase inhibition of SS.