Stromal transforming growth factor-beta 1 is crucial for reinforcing the invasive potential of low invasive cancer
Stromal transforming growth factor-beta 1 is crucial for reinforcing the invasive potential of low invasive cancer
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DOI：
10.1016/j.archoralbio.2014.03.017
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发表时间：
2014-07-01
影响因子：
3
通讯作者：
Chung, Won-Yoon
中科院分区：
文献类型：
--
作者：
Hwang, Young Sun;Park, Kwang-Kyun;Chung, Won-Yoon
目的：肿瘤细胞改变邻近间质的特性，在癌症进展过程中创造一个支持性微环境。通过体外和体内实验，验证间质转化生长因子-β1在增强低侵袭性肿瘤侵袭潜能中的作用。材料与方法：分离的NF或CAF与低侵袭性HSC-2细胞共同培养，观察间质转化生长因子-β1是否通过Transwell侵袭诱导PDPN的表达并影响原位移植瘤的生长。结果：转化生长因子-β1通过SMAD信号通路促进PDPN的表达和Transwell侵袭，并激活Src、P38丝裂原活化蛋白激酶和细胞外调节的蛋白激酶1/2。PDPN的诱导依赖于TβRII。结论：间质转化生长因子-β1信号可促进肿瘤细胞中PDPN的表达，从而促进肿瘤生长，并导致更具侵袭性的表型。(C)2014爱思唯尔有限公司。保留所有权利。
Objective: Tumour cells alter the characteristics of the adjacent stroma to create a supportive microenvironment during cancer progression. In vitro and in vivo experiments were carried out to verify the role of stromal TGF-beta 1 in reinforcing of the invasive potential in low invasive cancer.Materials and methods: Isolated NF or CAF was co-cultured with low invasive HSC-2 cells to evaluate whether stromal TGF-beta 1 induced PDPN expression by Transwell invasion and influenced tumour growth in orthotopic xenografts.Results: Stimulation by TGF-beta 1 promoted PDPN expression and Transwell invasion through SMAD signalling as well as activation of Src, P38 mitogen activated protein kinase and extracellular regulated kinase 1/2. PDPN induction was T beta RII-dependent. Tumour growth of HSC-2 OSCC in a mouse xenograft was intensified in the tumour CAP microenvironment.Conclusions: Stromal TGF-beta 1 signalling promoted PDPN expression in cancer cells, thereby enhancing tumour growth and leading to a more invasive phenotype. (C) 2014 Elsevier Ltd. All rights reserved.