The yeast SR protein kinase Sky1p modulates salt tolerance, membrane potential and the Trk1,2 potassium transporter
The yeast SR protein kinase Sky1p modulates salt tolerance, membrane potential and the Trk1,2 potassium transporter
复制标题
DOI：
10.1016/s0005-2736(02)00503-5
复制
发表时间：
2002-09-20
影响因子：
3.4
通讯作者：
Serrano, R
中科院分区：
文献类型：
--
作者：
Forment, J;Mulet, JM;Serrano, R
专用于SR蛋白磷酸化的蛋白激酶参与了mRNAs的加工和核输出。在这里，我们在酿酒酵母中展示了它们参与阳离子动态平衡的过程。单一酵母SR蛋白激酶Sky1p的零突变是活的，但对Na+、Li+、精胺、四甲铵、潮霉素B和Mn2+等多种有毒阳离子的耐受性增强。这种多效性表型与阳离子积累减少相关，表明膜电势降低。遗传分析和Rb+吸收测量表明，Sky1p调控酵母高亲和力K+吸收系统Trk 1，2，Trk 1，2是膜电位的主要决定因素。(C)2002 Elsevier Science B.V.保留所有权利。
Protein kinases dedicated to the phosphorylation of SR proteins have been implicated in the processing and nuclear export of mRNAs. Here we demonstrate in Saccharomyces cerevisiae their participation in cation homeostasis. A null mutant of the single yeast SR protein kinase Sky1p is viable but exhibits increased tolerance to diverse toxic cations such as Na+, Li+, spermine, tetramethylammonium, hygromycin B and Mn2+. This pleiotropic phenotype correlates with reduced accumulation of cations, suggesting a decrease in membrane electrical potential. Genetic analysis and Rb+ uptake measurements indicate that Sky1p modulates Trk 1,2, the high-affinity K+ uptake system of yeast and a major determinant of membrane potential. (C) 2002 Elsevier Science B.V. All rights reserved.